The 2x Phanta Max Master Mix (Vazyme, #P515) contains Phanta Max Super-Fidelity DNA Polymerase, dNTP, and an optimized buffer system. The amplification can start only with the addition of primer and template, thereby easing PCR setup and improving reproducibility. Protective agents in the 2x Phanta Max Master Mix enable the resistance to repeated freeze-thaw cycles. Amplification will generate blunt-ended products, which are compatible with ClonExpress II One Step Cloning Kit Series (i.e. Vazyme, #C112, #C113, #C115).

The Phanta Max Super-Fidelity DNA Polymerase (Vazyme, #P505) is a new generation superior enzyme based on Phanta DNA Polymerase for robust PCR with higher fidelity. The unique extension factor, specificity-promoting factors and plateau-inhibiting factor newly added to Phanta Max greatly improve its long-fragment amplification ability, specificity, and PCR yield. Phanta Max is capable of amplifying long fragments such as 40 kb λ DNA, 40 kb plasmid DNA, 20 kb genomic DNA and 10 kb cDNA. The amplification error rate of Phanta Max is 53-fold lower than that of conventional Taq and 6-fold lower than that of Pfu. In addition, Phanta Max has a good resistance to PCR inhibitors and can be used for direct PCR amplifications of bacteria, fungi, plant tissues, animal tissues, and even whole blood samples. Phanta Max contains two monoclonal antibodies inhibiting the 5" --> 3" polymerase activity and 3" --> 5" exonuclease activity at room temperature, which enable Phanta Max to perform highly-specific Hot-Start PCRs.Product Manual (pdf)

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Advantages

• Super Fidelity: 53-fold higher than Taq DNA Polymerase.
• Long Fragment: amplify fragments as long as 40 kb.
• Suitable for templates with high GC-content.
• Suitable for Direct-PCR using crude materials as templates: bacteria, fungi, whole blood, cultured cells, plant tissue lysate, food lysates, etc.

When order, Cold Shipping option have to be selected, or your order may be delayed.

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Selected Product Citations

1. Han X, et al. Mapping the Mouse Cell Atlas by Microwell-Seq. Cell, 2018. 172(5):1091-107.. Pubmed ID:29474909
2. Zhao Q, et al. Metabolic coupling of two small-molecule thiols programs the biosynthesis of lincomycin A. Nature, 2015, 518(7537):115-9.. Pubmed ID:25607359
3. Cheng X, et al. Pacer Mediates the Function of Class III PI3K and HOPS Complexes in Autophagosome Maturation by Engaging Stx17. Molecular Cell, 2017, 65(6):1029-43.. Pubmed ID:28306502
4. Tian Z, et al. An enzymatic [4+2] cyclization cascade creates the pentacyclic core of pyrroindomycins. Nature Chemical Biology, 2015, 11(4):259-65.. Pubmed ID:25730548
5. Lv M, et al. Characterization of a C3 Deoxygenation Pathway Reveals a Key Branch Point in Aminoglycoside Biosynthesis. Journal of the American Chemical Society, 2016, 138(20):6427-35.. Pubmed ID:27120352
6. Duan G, et al. Signal peptide represses GluK1 surface and synaptic trafficking through binding to amino-terminal domain. Nature Communications, 2018, 9(1): 4879.. Pubmed ID:30451858
7. Xu D, et al. USP14 regulates autophagy by suppressing K63 ubiquitination of Beclin 1. Genes & Development, 2016, 30(15):1718-30.. Pubmed ID:27542828

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Mechanism

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Storage

All components should be Store at -20°C.

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Components

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Related Products

1. Phanta Max Super-Fidelity DNA Polymerase (Vazyme #P505)
2. 2X Phanta Max Master Mix (Dye Plus) (Vazyme#P525)
3. ClonExpress Ultra One Step Cloning Kit (Vazyme, #C115)
4. ClonExpress II One Step Cloning Kit (Vazyme, #C112)
5. ClonExpress MultiS One Step Cloning Kit (Vazyme, #C113)
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