A83-01 is a selective inhibitor of ALK5 (12nM), ALK4 (45nM), and ALK7 (7.5 nM), and only weakly inhibits ALK1, 2, 3, and 6. It inhibits the TGF-β-induced epithelial-to-mesenchymal transition (EMT) via the inhibition of Smad2 phosphorylation. A83-01 has been used to generate rat and human iPS cells towards a mouse ES cell like self-renewal state.

Product Citation

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Technical information:

Chemical Formula:		C25H19N5S
CAS #:		909910-43-6
Molecular Weight:		421.52
Purity:		>98%
Appearance:		Pale yellow solid
Chemical Name:		3-(6-methylpyridin-2-yl)-N-phenyl-4-(quinolin-4-yl)-1H-pyrazole-1-carbothioamide
Solubility:		Up to 100 mM in DMSO

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Shipping Condition: The product is shipped in a glass vial at ambient temperature.Storage: For longer shelf life, store solid powder at 4^oC desiccated, or store DMSO solution at -20^oC.

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Reference:

1.	Tojo M, Hamashima Y, et al. The ALK-5 inhibitor A-83-01 inhibits smad signaling and epithelial-to-mesenchymal transition by transforming growth factor-beta. Cancer Science (2005), 96(11), 791-800.
2.	Li W, Wei W, et al. Generation of rat and human induced pluripotent stem cells by combining genetic reprogramming and chemical inhibitors. Cell Stem Cell (2009), 4(1), 16-19.

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Other Information:

Product Specification (pdf)MSDS (pdf)

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