Accordingtoliteratures^1-4,thesupplementofsmallmoleculecocktail(Repro-PACT)incellculturemediawillsimplifytheprocessandincreasetheproductivityingeneratingiPScells.Thisprocesscanbeeasilyscale-upsimplybyculturingmorecells.

Repro-PACTcanbeusedonmanycelltypes,includingprimaryskinfibroblasts,peripheralbloodmononuclearcells,endothelialcells,orkeratinocytes,andcanbeusedincombinationwithanyofthewidelyusedreprogramminggeneexpressionsystems(e.g.retro/lenti-viral,episomal,mRNA,orproteins)toenhancethereprogrammingtoiPScells.

---

Technicalinformation:

CocktailFormulation: 1000xDMSOstocksolution		DMSOsolutioncontains: 0.5mMofPD0325901, 0.5mMofA83-01, 3.0mMofCHIR99021,and 0.5mMofThiazovivin
Purity:		>98%(foreachcompound)

---

ShippingCondition:Theproductisshippedinaglassvialatambienttemperature.
Storage:StoreDMSOsolutionat-20^oC.
Usage:DiluteRepro-PACTat1:1000intoyourbasicESmediatomakeRepro-PACTmedia.

---

Reference:

1.	Linetal.(2009)AchemicalplatformforimproveinductionofhumaniPScells.NatureMethod6,805-808.
2.	Kawamataetal.(2010).Generationofgeneticallymodifiedratsfromembryonicstemcells.PNAS107,14223-14228.
3.	Zhuetal.(2010)ReprogrammingofhumanprimarysomaticcellsbyOCT4andchemicalcompounds.CellStemCell7,651-655.
4.	Yuetal.(2011)Efficientfeeder-freeepisomalreprogrammingwithsmallmolecules.PLoSONE6(3):e17557.

---

Cellagen的ChamQ SYBR qPCR预混液（无ROX）| Q321产品描述ChamQSYBR®qPCR预混液（Vazyme，＃Q321）由香槟Taq DNA聚合酶（Vazyme，＃P122）通过抗体修饰的热启动激活技术保护，是专为基于SYBR Green I的定量PCR（qPCR）设计的。ChamQSYBR®qPCR Master Mix的优化缓冲液系统中的独特因素（即促进特异性的Exactor）大大提高了其灵敏度和特异性。该混合物以2倍反应浓度制备，可直接用于鲁棒性低模板qPCR，具有高灵敏度，特异性和可靠性。优点特异性+敏感性的最佳组合。独特的抗体修饰的热启动taq，具有独特的特异性促进因子。Mg2 +和染料的最佳浓度。