P. aeruginosa Gyrase

Pseudomonas aeruginosa gyrase is a bacterial type II topoisomerase which can introduce negative supercoils into DNA. It is a target for both quinolone and coumarin drugs and can be used for screening potential antibacterial compounds. It is prepared by overexpressing the subunits in E. coli and then purified.

It is supplied as an A₂B₂ complex in Dilution Buffer. Store at -80 °C.

All enzyme is supplied with 5X concentrated Assay Buffer and Dilution Buffer which are also available separately. 1 U of gyrase will supercoil 0.5 µg relaxed pBR322 DNA in 30 minutes at 37 °C.

See technical documents below for more detailed information and lot specific activities.

Technical Documents

Cat No.	Product	Price	Quantity
PAG1001	P. aeruginosa Gyrase100 U	£110
PAG5002	P. aeruginosa Gyrase500 U	£405

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Kits are available containing everything required to perform supercoiling reactions and to test inhibitors. They contain the enzyme, relaxed DNA substrate and the Assay and Dilution buffers. 

Technical Documents

Cat No.	Product	Price	Quantity
PAS001	P.aeruginosa Gyrase Supercoiling Assay Kit 1100 U of P. aeruginosa gyrase and 50 µg of relaxed DNA	£191
PAS002	P.aeruginosa Gyrase Supercoiling Kit 2500 U of P. aeruginosa gyrase and 50 µg of relaxed DNA	£725

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