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商品描述
| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name | Normetanephrine ELISA kit - Plasma - Fast-Track |
| Description | In vitro diagnostics enzyme immunoassay (ELISA) for the fast determination of free normetanephrine (normetadrenaline) in plasma samples. |
| Labels | IVD, CE |
| Format | 96-well plate |
| Samples | plasma |
| Sample volume | 500 µL |
| Cross specie-reactivity | React with all species |
| Standard range | 0 / 48 - 4 800 pg/ml |
| Sensitivity | 23 pg/ml |
| Specificity | No significant cross-reactivity was observed with Normetanephrine analogs such as Metanephrine, 3-methoxytyramine, Adrenaline, Noradrenaline, Dopamine, VMS, HVMS, L-Dopa, L-Tyrosine, Tyramine and Acetaminophen. |
| Assay time | Sample preparation and acetylation 30 min and ELISA overnight |
| Storage | Store at 2-8°C for to 6 months. |
| Datasheets | Instructions for use , Material safety datasheet |
Key steps
| Sample collection & storage | EDTA- or citrate-plasma sample only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 - 8°C; for longer periods (up to 6 months) at - 20°C. |
| Sample preparation | Sample precipitation and acetylation (30min) |
| ELISA | Normetanephrine antiserum overnight incubation, revelation and read steps (1h). |
| Detailed protocol | Download instructions for use |
References
- Manz B et al. Development of enantioselective immunoassays for free plasma metanephrines. Ann N Y Acad Sci. 2004 Jun;1018:582-7.
- Gao YC et al. Comparison of free plasma metanephrines enzyme immunoassay with (131)I-MIBG scan in diagnosis of pheochromocytoma. Clin Exp Med. 2008 Jun;8(2):87-91.
- Procopiou M et al. Evaluation of an enzyme immunoassay for plasma-free metanephrines in the diagnosis of catecholamine-secreting tumors. Eur J Endocrinol. 2009 Jul;161(1):131-40.
- Christensen TT et al. Comparison of plasma metanephrines measured by a commercial immunoassay and urinary catecholamines in the diagnosis of pheochromocytoma. Scand J Clin Lab Invest. 2011 Dec;71(8):695-700.
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