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商品描述
| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name | Serotonin ELISA kit - Research |
| Description | Enzyme immunoassay (ELISA) for the ultra-sensitive determination of Serotonin in biological samples. Research use only. |
| Format | 96-well plate |
| Samples | 1 - 100 µl - any biological sample |
| Cross-species reactivity | React with all species |
| Standard range | 0 / 0.015 - 2.5 ng/ml |
| Sensitivity | 5 pg/ml |
| Specificity | No significant cross-reactivity was observed with Serotonin analogs such as Tryptamine, Melatonin, 5-Hydroxyindole acetic acid, Phenylalanine, Histidine, Tyramine and 5-Hydroxytryptophan. |
| Assay time | 30 min + overnight |
| Storage | Store at 2-8°C for up to 6 months. |
| Datasheets | Instructions for use , Material safety datasheet |
Key steps
| Sample preparation | Sample acylation (30min) |
| ELISA | Serotonin antiserum incubation (overnight), revelation and read steps (1h). |
| Detailed protocol | Download instructions for use |
References
- Product citation: Sabti et al. Elucidation of the Molecular Mechanism Underlying Lippia citriodora(Lim.)-Induced Relaxation and Anti-Depression. Int J Mol Sci. 2019 Jul 20
- Product citation: Lau et al. The presence of serotonin in cigarette smoke – a possible mechanistic link to 5-HT-induced airway inflammation. Free Radic Res. 2016;50
- Product citation: Pham et al. Ketamine treatment involves medial prefrontal cortex serotonin to induce a rapid antidepressant-like activity in BALB/cJ mice. Neuropharmacology. 2017 Jan;112
- Valiyaveettil M et al. Contribution of systemic factors in the pathophysiology of repeated blast-induced neurotrauma. Neurosci Lett. 2013 Feb 28;539:1-6.
- Damen R et al. The stimulatory adenosine receptor ADORA2B regulates serotonin (5-HT) synthesis and release in oxygen-depleted EC cells in inflammatory bowel disease. PLoS One. 2013 Apr 26;8(4):e62607.
- Kaushal N et al. Pharmacological evaluation of SN79, a sigma (σ) receptor ligand, against methamphetamine-induced neurotoxicity in vivo. Eur Neuropsychopharmacol. 2013 Aug;23(8):960-71.
- Rokstad AM et al. Microencapsulation of small intestinal neuroendocrine neoplasm cells for tumor model studies. Cancer Sci. 2012 Jul;103(7):1230-7.
- Chin A et al. The role of mechanical forces and adenosine in the regulation of intestinal enterochromaffin cell serotonin secretion. Am J Physiol Gastrointest Liver Physiol. 2012 Feb 1;302(3):G397-405.
- van den Buuse M. Exploring the role of 5-HT1A receptors in the regulation of prepulse inhibition in mice: implications for cross-species comparisons. ACS Chem Neurosci. 2013 Jan 16;4(1):149-60.
- ...
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