The T7 mScriptTM Standard mRNA Production System provides all enzymes and enzyme-related reagents for making 5 -capped, 3 -polyadenylated mRNA. This mScript Kit includes modules for (i) in vitro transcription of linear double-stranded DNA templates using the mScript T7 Enzyme Solution, the canonical nucleotides ATP, CTP, GTP and UTP, (ii) enzymatic capping of the RNA using ScriptCapTM Capping Enzyme System (for making mRNA with a Cap 0 cap structure), (iii) ScriptCapTM 2 -O-Methyltransferase (for optionally making mRNA with a Cap 1 cap structure) and (iv) A-PlusTM Poly(A) Polymerase for adding a 3 -poly(A) tail.
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