PRODUCT SPECIFICATIONS

PRODUCT# 40002:  Recombinant HBV Surface Antigen Ad (E.coli)

SPECIFICATIONS                                                               APPLICATIONS

Concentration:	See vial	Diagnostics/Elisa/WB
Mass/vial:	1mg	Immunization
Volume/vial:	1ml	T-Cell Activation
Diluent:	10mMNa-PO4, 50mM NaCl, 50% Glycerol, pH 7.2
Purity:	>92%
Stabilizer:	None
Preservative:	None
Storage:	-75C
Physical State:	Aqueous Solution
Stability:	24  Months at -75oC
Lot #: 10F24V

 

DESCRIPTION: Recombinant HBV surface protein Ad  expressed in the E.coli Expression system.

PURIFICATION: Proprietary. Purity > 92 % as determined by SDS_PAGE, reduced. MW: aapox. 24kD

SPECIFICITY: Strongly binds to HBV converted human serum polyclonal antibodies as determined by  ELISA and Western ELISA. Protein is suitable for lateral flow diagnostic assays.

BIOLOGICAL ACTIVITY: Not determined.

APPLICATION AND INSTRUCTIONS FOR USE

Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

ELISA and Western ELISA require 0.1-1.0µg protein depending on the nature and affinity of the secondary  detection reagent.  Studies with HBV convereted human sera are performed in 1:200 to 1:10,000 antibody y dilution range.

Glossary

Gene and Gene Products

Structural Proteins: Structural proteins – the products of gag, pol and env genes, which are essential components of the retroviral particle.

 

Regulatory Proteins: Regulatory proteins – tat and rev proteins of HIV/SIV and tax and rex proteins of HTLVs; essential for viral expression in infected cells.

 

Accessory Proteins: Accessory proteins – additional (non-regulatory) virion – and non virion-associated proteins produced by HIV/SIV retroviruses: vif, vpr, vpu, vpx, and nef. Although, the accessory proteins are not necessary for viral propagation in tissue culture, they have been conserved in the different isolates; this conservation and experimental observations suggest that their role in vivo is very important. 

 

gag

gag – group-sepecifc antigens or capsid proteins; the precursor is the p55 myristoylated protein, which is processed to p17 (Matrix) p24 (Capsid) and p7 (NucleoCapsid) proteins by the viral protease. Other small proteins are generated from the gag polyprotein.

 

pol

pol – (p66) generates the viral enzymes protease (p11), reverse transcriptase (p51), endonuclease and integrase (p32) after the processing of a gag-pol precursor polyprotein by the viral protease; gag-pol precursor is produced by ribosome frameshifting. 

 

env

env – viral glycoproteins produced as a precursor (gp160) and processed to the external glycoprotein (gp120) and the transmembrane glycoprotein (gp41). The mature proteins are held together by noncovalent interactions; as a result substantial amount of gp120 is released extracellularly. The external glycoprotein (gp120) contains the binding site for the CD4 receptor. 

 

tat

tat – transactivator of HIV gene expression; one of the two necessary viral regulatory factors (tat and rev) for HIV gene expression. Two forms are known, tat-1 exon (minor form) of 72 amino acids, and tat-2 exon (major form) of 86 amino acids. The electrophoretic mobility of these two forms in SDS gels is anomalous; they are approximately 16 kD and 14 kD in weight. Low levels of both proteins are found in persistently infected cells. tat is localized primarily in the nucleolus/nucleus; it acts by binding to the TAR RNA element and activating transcription from the LTR promoter. Post-transcriptional effects of tat have been postulated. 

 

rev

rev – the second necessary regulatory factor for HIV expression. A 19 kD phosphoprotein localized primarily in the nucleolus/nucleus, rev acts by binding to RRE and promoting the nuclear export, stabilization and utilization of the viral mRNAs containing RRE.

 

vif

vif – viral infectivity factor, typically 23 kD; required for the efficient transmission of cell-free virus in tissue culture. In the absence of vif, the produced viral particles are defective, while the cell-to-cell transmission of virus is not affected significantly. It has been reported that the cellular localization is in the Golgi (vif is not found in the virion). 

 

nef

nef – approximately 27 kD non-virion protein found in the cytoplasm of infected cells. Potentially myristoylated and associated with the inner plasma membrane. One of the first HIV proteins to be produced in the infected cells, it is the most immunogenic of the accessory proteins and may be used in the future for diagnosis and staging of the disease. NEF is dispensable and probably suffers counter-selection during ex vivo viral propagation in vivo. Recent evidence suggests that SIV nef is required for viral propagation in vivo.

 

vpr

vpr – virion-associated protein of unknown function found in HIV-1, HIV-2, SIVmac, and SIVmnd; typically 15 kD. May be homologous to vpx. Also called “rap” for rapid.

 

vpu

vpu – protein that promotes extracellular release of viral particles. Found only in HIV-1. Integral membrane phosphoprotein of 16kd; similar to M2 protein of influenza virus. It may be involved in env maturation. It is not found in the virion. 

 

vpx

vpx – virion protein of 12 kD found only in HIV-2 infection. (vpx may have some homology with vpr).

Related research paper:

immunodx重组抗原重组抗原是从实验室而不是从其生物学来源人工制造的抗原。就像天然抗原一样，它们能够使免疫系统发挥作用，从而使人体免受伤害。 并非市场上所有的重组抗原都是相同的。来自我们的  ImmunoDX具有极高的质量，并且与天然同类产品高度相似。 为了生产理想用于各种研究和诊断目的的重组抗原，我们使用了一些最具创新性和可靠性的蛋白质工程方法。由于一切都在我们的工厂中进行，因此我们保证了重组抗原纯度，特异性和生物活性的高水平标准。 我们的重组抗原以相同的方式非常接近天然抗原。但是，它们的生产不受诸如高需求和高税率等约束因素的影响。ImmunoDX可以以少量成本提供大量一流的重组抗原。 ImmunoDX的重组抗原非常适合  各种各样的应用，包括用于产生商业用途抗体的免疫程序。 多年来，ImmunoDX一直专门从事与关节炎，癌症，结核病，艾滋病等相关的研究和诊断的特殊生物学产品的生产。我们还提供许多其他相关的产品和服务。