PRODUCT SPECIFICATIONS

PRODUCT # 1108-B:  Biotin Murine Anti-Nef HIV-1 mAb IgG

SPECIFICATIONS                                         APPLICATIONS

Mass/vial: Approximately 50µg                    IgG Elisa/Westerns

Volume: 50µl                                                  FACS

Diluent: PBS                                                 Immunohistocytology

Stabilizer: 1% BSA

Preservative: 0.1% Azide

Storage: 4oC

Physical State: Liquid  

B/P Ratio: 5-10

Stability: At least 6 months at 4oC.

Minimum Dilution: 1 : 2000                                    

DESCRIPTION: Biotin conjugated Murine anti-Nef HIV-1 mAb.

PURIFICATION: Highly purified mAb IgG (>95% pure) was used for Biotin conjugation and the conjugate purified by gel exclusion chromatography.

SPECIFICITY: This biotin conjugated mAb binds to native and recombinant HIV-1 Nef in ELISA and Western ELISA.

BIOLOGICAL ACTIVITY: None

APPLICATION AND INSTRUCTIONS FOR USE

Recommended dilutions for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

ELISA may be performed in 1 : 2000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents.  Western ELISA may be performed in 10-100pg Nef range with 1:500 dilution of the conjugate.

immunodx重组抗原重组抗原是从实验室而不是从其生物学来源人工制造的抗原。就像天然抗原一样，它们能够使免疫系统发挥作用，从而使人体免受伤害。 并非市场上所有的重组抗原都是相同的。来自我们的  ImmunoDX具有极高的质量，并且与天然同类产品高度相似。 为了生产理想用于各种研究和诊断目的的重组抗原，我们使用了一些最具创新性和可靠性的蛋白质工程方法。由于一切都在我们的工厂中进行，因此我们保证了重组抗原纯度，特异性和生物活性的高水平标准。 我们的重组抗原以相同的方式非常接近天然抗原。但是，它们的生产不受诸如高需求和高税率等约束因素的影响。ImmunoDX可以以少量成本提供大量一流的重组抗原。 ImmunoDX的重组抗原非常适合  各种各样的应用，包括用于产生商业用途抗体的免疫程序。 多年来，ImmunoDX一直专门从事与关节炎，癌症，结核病，艾滋病等相关的研究和诊断的特殊生物学产品的生产。我们还提供许多其他相关的产品和服务。