Product Specifications:
Item# 7001 Recombinant Human T-Cell Receptor sCD4
Concentration: See vial
Mass/vial: 0.1mg
Volume/vial: 1ml
Diluent: PBS
Purity: >98%
Stabilizer: None
Preservative: None
Storage: -75°C
Physical State: Frozen Liquid
Stability: At least 24 months at -75°C
Applications: T-Cell Research,Human Diagnostics, HIV-1 Neutralization, Drug Screening
Description: Full length recombinant human T-Cell receptor sCD4 produced in the baculovirus expression system. The full length protein contains V1 through V3 domains of CD4.
Purification:This protein is purified by immunoaffinity and gel exclusion chromatography to >98% purity as determined by SDS PAGE, reduced.
Molecular weight: Approximately 55kD.
Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity in ELISA and Western ELISA.
Biological Activity: This protein binds to native and recombinant HIV-1 gp120 and HIV-2 gp105 and neutralizes HIV-1 infectivity in CD4-bearing, HIV-1 infected cell cultures and HIV infected PCMBs.
Application and Instructions for use:
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
1. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the secondary detector reagent(s).
2. Recommended concentration for virus neutralization assays are in 1-10µg/ml of the HIV-1 infected culture.
3. Drug screening assays are performed with 100-500ng of solid phase sCD4 and gp120 binding is determined in the presence of inhibitors either indirectly using murine anti-gp120-peroxidase conjugate (catalog# 1121-P) or directly using HIV-1 rgp120-peroxidase conjugate (catalog# 1001-P).
immunodx重组抗原重组抗原是从实验室而不是从其生物学来源人工制造的抗原。就像天然抗原一样，它们能够使免疫系统发挥作用，从而使人体免受伤害。 并非市场上所有的重组抗原都是相同的。来自我们的 ImmunoDX具有极高的质量，并且与天然同类产品高度相似。 为了生产理想用于各种研究和诊断目的的重组抗原，我们使用了一些最具创新性和可靠性的蛋白质工程方法。由于一切都在我们的工厂中进行，因此我们保证了重组抗原纯度，特异性和生物活性的高水平标准。 我们的重组抗原以相同的方式非常接近天然抗原。但是，它们的生产不受诸如高需求和高税率等约束因素的影响。ImmunoDX可以以少量成本提供大量一流的重组抗原。 ImmunoDX的重组抗原非常适合 各种各样的应用，包括用于产生商业用途抗体的免疫程序。 多年来，ImmunoDX一直专门从事与关节炎，癌症，结核病，艾滋病等相关的研究和诊断的特殊生物学产品的生产。我们还提供许多其他相关的产品和服务。
