PRODUCT SPECIFICATIONS
ITEM# 4004: Recombinant HCV NS3 Protein (E coli)
SPECIFICATIONS
Concentration: see vial
Mass/vial: see vial
Diluent: PBS, pH 7.4, 0.05% Sarcosyl.
Purity: >95%
Stabilizer: None
Preservative: None
Storage: -75o C
Stability: At least 12 months at -75o C
DESCRIPTION: Hepatitis C recombinant NS3 protein expressed as a fusion protein produced in the E.coli expression system. Molecular Size: approx: 101kD.
APPLICATIONS: In vitro-HCV Diagnostics, T-cell Activation, Elisa western.
PURIFICATION: HCV rNS3 is purified by ion-affinity chromatography and preparative electrophoresis methods to >95% purity as determined by SDS-PAGE, reduced.
SPECIFICITY: This protein binds to murine monoclonal antibodies and shows strong reactivity with HCV converted human serum polyclonal antibodies in ELISA and Western ELISA.
BIOLOGICAL ACTIVITY: Not determined.
APPLICATION AND INSTRUCTIONS FOR USE
Recommended concentrations for use are approximate values. Dilute the antigen preparation at least 10 to 20-fold in appropriate buffers for solid phase antigen-antibody titration assays. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. HCV NS3 protein shows relatively strong binding specificity for HCV-specific human antibodies. ELISA and Western ELISA require protein in 10ng range depending on the nature and affinity of the secondary detection reagent. HCV converted human serum polyclonal antibodies yield titers of 1:10,000 or greater at 100ng of immobilized protein under standard ELISA conditions.
immunodx重组抗原重组抗原是从实验室而不是从其生物学来源人工制造的抗原。就像天然抗原一样，它们能够使免疫系统发挥作用，从而使人体免受伤害。 并非市场上所有的重组抗原都是相同的。来自我们的 ImmunoDX具有极高的质量，并且与天然同类产品高度相似。 为了生产理想用于各种研究和诊断目的的重组抗原，我们使用了一些最具创新性和可靠性的蛋白质工程方法。由于一切都在我们的工厂中进行，因此我们保证了重组抗原纯度，特异性和生物活性的高水平标准。 我们的重组抗原以相同的方式非常接近天然抗原。但是，它们的生产不受诸如高需求和高税率等约束因素的影响。ImmunoDX可以以少量成本提供大量一流的重组抗原。 ImmunoDX的重组抗原非常适合 各种各样的应用，包括用于产生商业用途抗体的免疫程序。 多年来，ImmunoDX一直专门从事与关节炎，癌症，结核病，艾滋病等相关的研究和诊断的特殊生物学产品的生产。我们还提供许多其他相关的产品和服务。
