PRODUCT SPECIFICATIONS

 

ITEM # 1099:  Recombinant HIVO gp41 Envelope Protein (E.coli)

                                                           

   SPECIFICATIONS                                                          

  Concentration:                 See vial                                                                                                                                                         

  Mass/vial:                          1mg 

  Volume/vial:                     see vial                                                       

 Diluent:                            10 mM Tris, pH 8,<0.02%SDS                                     Purity:                                >98%                             

Stabilizer:                          None                                                                     

Preservative:                     None                                                                     

Storage:                             -75oC

 Physical State:                  Frozen Liquid

 Stability:                            At least 24 months at -75oC.           

 DESCRIPTION: Truncated  Recombinant HIV O envelope protein gp41 produced in  the E.coli Expression System. Truncated gp41 protein mol.wt: 25kD.

APPLICATION:  In-Vitro Diagnostics, Immunization,   T-Cell Activation.

PURIFICATION: This protein was purified by affinity chromatography and by preparative electrophoresis to >98% purity as determined by SDS-PAGE, reduced.

 SPECIFICITY: This protein binds to HIVO converted human serum polyclonal antibodies in ELISA  and Western  Elisa.

 BIOLOGICAL ACTIVITY:  Not done

 APPLICATION AND INSTRUCTIONS FOR USE

 Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

 ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent.  Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions.

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