Product Specifications:

Item # 1007-F: Fluorescene vif HIV-1

 

Mass/vial: At least 50µg vif  

 

Diluent: 10mM Tris, pH 8.0                                  

 

Stabilizer: None                                                       

 

Preservative: None                                                       

 

Storage: -75°C                                                                             

 

F/P Ratio: 2 - 4

 

Stability: At least 6 months at -75°C

 

Minimum Dilution:   1 : 50

 

Applications: Dot Blots, FACS, Immunohistocytology, Immunoflorecence

 

Description: E.coli derived vif (HIV-1 ) conjugated to FITC.

 

Purification: Fluorescene conjugated vif was purified by gel exclusion chromatography and UF concentrated.

 

Specificity: This fluorescene conjugated vif binds to murine monoclonal antibodies of defined epitope specificity.

 

Biological Activity: ND

 

Application and Instruction for use:

Recommended dilutions for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Direct Immunofluorescent staining of CD4 bearing cells may be performed in 1 : 50 dilution range.  Dot Blot assays with purified anti-gp160 monoclonal/polyclonal antibodies may be performed at a minimum dilution of 1 : 100.

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