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蚂蚁淘在线 / 品牌中心 / Cell Biolabs品牌新闻

CRL-2579 RKO-AS45-1 人结肠癌转基因细胞

作者： 时间：2024-09-20 点击量：

货号： CRL-2579
数量： 大量
是否是肿瘤细胞： 0
CRL-2579 RKO-AS45-1 人结肠癌转基因细胞 的详细介绍CRL-2579 RKO-AS45-1 人结肠癌转基因细胞
ATCC® Number: CRL-2579™    Price:
Designations: RKO-AS45-1
Depositors:  MC Hollander, AJ Fornace
Biosafety Level:2 [Contains CMV viral sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties:adherent
Organism:Homo sapiens (human)
Morphology:epithelial 
Source:Organ: colonDisease: carcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Oncogene:p53 + (underexpressed)
Comments:The RKO-AS45-1 cell line was generated from the colon carcinoma RKO (ATCC CRL-2577) cell line by transfection with the open reading frame portions of human cDNAs for GADD45 cloned into the expression vector pCMV. [51464]The cells contain a stably integrated cytomegalovirus (CMV) promotor driven expression vector. The cells overexpress Gadd45 RNA and protein.The GADD45 gene is p53-regulated and the Gadd45 protein interacts with proteins involved in both cell-cycle regulation and DNA repair.The RKO-AS45-1 cell line has reduced ability to repair U.V.-induced DNA damage. [51464]The RKO-AS45-1 cell line can be used together with its parental cell line, RKO (ATCC CRL-2577) to investigate the effects of Gadd45 on DNA repair, apoptosis and drug sensitivity.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:10 is recommendedMedium Renewal: Every 2 to 3 daysRemove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003recommended serum:ATCC 30-2020parental cell line:ATCC CRL-2577
References:51464: Smith ML, et al. Antisense GADD45 expression results in decreased DNA repair and sensitizes cells to u.v.-irradiation or cisplatin. Oncogene 13: 2255-2263, 1996. PubMed: 895099353491: Smith ML, et al. Interaction of the p53-regulated protein Gadd45 with proliferating cell nuclear antigen. Science 266: 1376-1380, 1994. PubMed: 797372753492: Bae I, et al. An abnormality in the p53 pathway following gamma-irradiation in many wild-type p53 human melanoma lines. Cancer Res. 56: 840-847, 1996. PubMed: 8631022

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