FGF R1 α (IIIc) - Fc Chimera
Symansis" FGF R1 alpha (IIIc) - Fc Chimerahcx has N-linked oligosaccharides, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F, and probably O-linked oligosaccharides. Symansis" FGF R1 alpha (IIIc) - Fc Chimerahcx contains 35-50% carbohydrate by weight. Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein. 1D SDS-PAGE of Symansis" hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation. 1. Eswarakumar VP, et al. (2005) Cytokine Growth Factor Rev. 16: 139-49.2. Ezzat S & Asa SL (2005) Horm Metab Res. 37: 355-60. | |  |
