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Expansion of mesenchymal stem cells (MSCs) in serum-free media such as StemRD’s MesenGro® (now available as StemGro® hMSC Medium from Corning) is a critical step towards clinical application.It has been found that isolation of primary MSCs from human tissues in serum-free media suffers from low efficiency due to a lack of cell adhesion proteins.MSCprime® has been developed to resolve this deficiency. MSCprime® is a serum-free (no human or animal serum added) and chemically-defined (all ingredients are either purified or recombinant).MSCprime® is available in 3 package sizes, and frozen in concentrated solution (25 X)Expected Results:MSCprime®-isolated homogeneous/smaller MSCs subsequently expanded in MesenGro®:
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Fig.1 shows MSCs isolated by MSCprime® expand faster in MesenGro® with more desirable morphology than in FBS in Fig.2. |
Product Sheet
Stemrd的Y-27632,(1R,4r)-4-((R)-1-氨乙基)-N-(吡啶-4-基)环己烷-羧酰胺-2HCl,是一种Rho相关激酶(ROCK)抑制剂。Y27632已被证明可以防止凋亡,并提高解离的人类胚胎干(ES)细胞的存活率和克隆效率,而不会影响其多能性。