SBI EXO-FBS-50A-1-不含外泌体的胎牛血清大量现货供应，2600元一瓶；

System BiosciencesSystem Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创欣生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒的所有相关产品、质粒、试剂盒及相关配套试剂和慢病毒延伸产品如IPS细胞多功能性诱导试剂盒和RNAi筛选文库。

概观

避免无意中研究牛外泌体

胎牛血清或FBS是许多类型细胞培养基的重要组成部分。但对于有兴趣从培养细胞中分离外泌体的研究人员来说，标准FBS可能会引入不需要的并发症 - 牛外泌体，这可能会导致严重的背景问题或干扰功能研究。这就是为什么SBI开发了Exo-FBS，我们的砖利外泌体耗尽的FBS。

• 去除外泌体大小的囊泡
• 非常低水平的CD63阳性牛外泌体
• 无法检测到的牛miRNA水平
• 可比增长率为标准FBS
• 可与标准FBS互换（在DMEM或RPMI中添加10％）

支持数据

高品质和卓越的性能

Exo-FBS支持强大的细胞生长（图1），大大降低了牛外泌体（图2）和牛miRNA（图3）的水平。补充有Exo-FBS的培养基中的细胞生长类似于补充有标准FBS的培养基中的细胞生长（图1）。

Exo-FBS支持比竞争对手更强大的细胞生长健康

图1. Exo-FBS支持比G公司外泌体耗尽的FBS更强大的细胞生长。HepG2细胞在含有标准FBS（对照），Exo-FBS或Company G的外来体耗尽的FBS的培养基中生长。在一次和两次传代后显示数据，并说明Exo-FBS提供比公司G产品更多数量的活细胞（左图，使用CCK-8测定评估的活力）和更健康的细胞形态（右图）。

图2.牛α-CD63 ELISA显示Exo-FBS中低水平的外泌体CD63是外来体特异性标记物。标准FBS和Exo-FBS的α-CD63 ELISA在Exo-FBS中显示非常低水平的CD63，支持NTA数据，其显示Exo-FBS中外来体大小的颗粒数量低（图1）。使用等体积（50μl）的标准FBS或Exo-FBS耗尽的培养基补充物，并将绘制的结果归一化至标准FBS的信号水平。

图3.qPCR测定显示Exo-FBS中不可检测水平的牛外泌体miRNA。虽然标准FBS含有可扩增的miRNA（测试的72个单独miRNA中的12个，左图），但Exo-FBS显示无可扩增的miRNA（右图）。用Trizol提取方法处理标准FBS和Exo-FBS培养基补充物（4ml）以回收外来体RNA。将RNA转化为cDNA，并使用SBI的QuantiMir系统通过qPCR测量72个单独的牛microRNA。

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