The PromoKine 3D Cell Culture Viability Assay Kit provides an easy-to-use, high-throughput and sensitive method for screening, characterizing and studying cell viability, and cytotoxicity in three-dimensional cell cultures. The standardized fluorometric method reliably quantifies viable cells detecting as low as 50 viable cells per well. The measured fluorescence intensity at Ex/Em = 485/530 nm is directly proportional to the number of viable cells. The complete kit comes with an optimized and gentle non-enzymatic dissociation solution for the recovery of viable and dead cells from spheroids in matrices and scaffolds.