ATP-Glo™BioluminometricCellViabilityAssayoffersahighlysensitiveassayforquantifyingATP.ThehomogeneousassayprocedureinvolvesasingleadditionofATP-Glo™DetectionCocktaildirectlytocellsculturedinaserum-supplementedmedium.Itisnotnecessarytoremovecellculturemediumorwashcellsbeforeaddingthereagent.
BecauseATPisanindicatorofmetabolicallyactivecells,thenumberofviablecellscanbeassessedbasedontheamountofATPavailable.ThisATPdetectionkittakesadvantageoffireflyluciferase’suseofATPtooxidizeD-LuciferinandtheresultingproductionoflightinordertoassesstheamountofATPavailable.
TheATP-Glo™kitcanbeusedtodetectaslittleasasinglecellor0.01picomoleofATP.Thesignalproducedislinearwithin6ordersofmagnitude.ByrelatingtheamountofATPtothenumberofviablecells,theassayhaswideapplications,rangingfromthedeterminationofviablecellnumberstocellproliferationtocellcytotoxicity.
Pleasenote:ATP-Glo™isaflash-typeluminescenceassay.Theluminescencesignalgeneratedisstableforupto1minute.Thisassayisdesignedforindividualsampledetectionbyusingaluminometerinasinglesampleformatoraluminometerwithaninjectorin96-wellplateformat.
KitComponents:
- D-Luciferin
- Fireflyluciferase
- ATP-Glo™assaybuffer
- 2mMATPstandard
Features:
- HighSensitivityandExtendedLinearity:Detectfromasinglecelltoseveralmillioncellswithexcellentlinearity
- Simple:Asingle-stephomogenousassay
- Versatile:DetectATPamountorquantifynumberoflivecells
MoreLuciferaseKits
| Catalognumber | Product | Description |
|---|---|---|
| 30085 | FireflyLuciferaseAssayKit2.0 | Flash-typefireflyluciferaseassaykit. |
| 30075 | FireflyLuciferaseAssayKit(Lyophilized) | Fireflyluciferaseassaykitwithlyophilizedassaybufferforeconomicalroomtemperatureshippingandconvenientstorageat-20°C. |
| 99923 | 5XFireflyLysisBuffer | ExtralysisbufferforusewithFireflyLuciferaseAssayKits,onlyrequiredifusingwellsizeslargerthan24-well. |
| 30028 | Steady-Luc™FireflyHTSAssayKit | Glow-typefireflyluciferaseassaywithsignalhalf-lifeofabout3hoursforhigh-throughputscreeninginmulti-wellplates. |
| 30028L | Steady-LucFireflyHTSAssayKit(Lyophilized) | Steady-Luckitwithlyophilizedassaybufferforeconomicalroomtemperatureshippingandconvenientstorageat-20°C. |
| 30082 | RenillaLuciferaseAssayKit2.0 | Flash-typeRenillaluciferaseassaykit. |
| 99912 | 5XPassiveLysisBuffer | ExtralysisbufferforusewithRenillaLuciferaseAssayKits,onlyrequiredifusingwellsizeslargerthan24-well. |
| 30081 | FireflyandRenillaLuciferaseSingleTubeAssayKit | AssaykitformeasuringfireflyandRenillaactivitiessequentiallyinthesamesampleinasingletube. |
| 99821-100mL | 1XPassiveLysisBuffer2.0 | ExtralysisbufferforusewithFirefly&RenillaSingleTubeAssayKits,onlyrequiredifusingwellsizeslargerthan24-well. |
| 30020 | ATP-Glo™BioluminometricCellViabilityAssayKit | Flash-typeluminescence-basedassayformeasuringcellviabilityorATPconcentration. |
| 22003 | MiniCellScrapers | Mini-cellscrapers,usefulforlysingcellsin96-well,48-well,or24-wellplates. |
References
1.JBioluminChemilumin10,29(1995).
2.AnalBiochem175,14(1988).
3.BiotechnolBioeng42,30(1993).
4.J.BioluminChemilumin6,193(1991).
5.BiochemJ295,165(1993).
6.J
ImmunolMeth160,81(1993).
