The cell permeable DMNPE-caged Luciferin is as a bioluminescent luciferase substrate used to measure intracellular functions since it readily crosses cell membranes. It is a D-luciferin ester analogue of luciferin.Once the caged luciferin is inside the cells, active luciferin can be released by the action of endogenous intracellular esterases or by a flash of UV light.

This implies:

1. A more efficient delivery of luciferin into intact living cells & improved sensitivity analysis of in vivo luciferase assays
2. A long-term measurement of luciferase activity since hydrolysis by esterases allows a continuous supply of active Luciferin

 

DMNPE-caged luciferin appears a good alternative to D-Luciferin classically used to measure luciferase activity in live cells. Indeed, the difficulty to deliver D-Luciferin into living cells renders in vivo quantification limited to a certain extent - for example, permeability issues at neutral pH.

Storage: -20°C and protect from light

Shipping Conditions: Room temperature

DMNPE-caged luciferin is provided in an amber bottle under nitrogen. 

 

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OZ Biosciences的VaxOZ佐剂非常适合作为金标准参照物和任何免疫实验。也可以使用您自己的免疫刺激剂来定制它们。当前，佐剂仍然是提高常规和下一代疫苗效率的最重要策略。遗传佐剂是与特定抗原结合使用的免疫学试剂，可增强免疫反应。直接后果是与仅使用抗原相比，会产生更多的抗体和更长的免疫力，从而使疫苗所需的抗原剂量降至最低。特别是，基于DOTAP（1,2-油酰基--3-三甲基铵-丙烷）的阳离子脂质可诱导树突状细胞成熟，然后运输至引流淋巴结以激活抗原特异性T细胞。疫苗佐剂OZ生物科学VaxOZ佐剂是体内制造和质量控制的产品，仅用于研究目的。- AlumVax氢氧化物 / 磷酸盐，两个古典铝基佐剂- IFAVax， 不完全弗氏佐剂- CFAVax，完全弗氏佐剂- SqualVax，一个纳米油包水乳液-  CaLiVax-DOTAP， 对于脂质体介导的DNA或蛋白质疫苗基于阳离子脂质的组合物。