OZ Biosciences SEAP Assay Kit (Secreted Alkaline Phosphatase) is a colorimetric assay for sensitive quantification of SEAP in culture medium from  transfected cells or tissues. Alkaline phosphatase cleaves the colourless 4-nitrophenylphosphate (pNPP) substrate to give a yellow p-nitrophenolate product in alkaline solutions. It is commonly used as a reporter gene expression and can be accurately quantified. It is a truncated form of placental alkaline phosphatase (GPI-anchored protein).

The kit contains sufficient reagents to perform 500 assays in 96-well plate format.

• Designed to detect SEAP reporter gene expression

• Fast and easy analysis

• Colorimetric – measure with a standard spectrophotometer or an ELISA reader (405 nm)

• Multiple samples can be analyzed in low volumes

• Convenient and economical packaging: no tablet, no powder

 

Sizes:pNPP-containing Assay Buffer component A: 10 x 1 mL

Reaction Buffer #1 component B: 125 mL

Reaction Buffer #2 component C: 125 µL

Dilution Buffer component D: 50 mL

 

Storage: -20°C for component A  / +4°C for components B, C and D

Shipping Conditions: Room Temperature

 

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OZ Biosciences的VaxOZ佐剂非常适合作为金标准参照物和任何免疫实验。也可以使用您自己的免疫刺激剂来定制它们。当前，佐剂仍然是提高常规和下一代疫苗效率的最重要策略。遗传佐剂是与特定抗原结合使用的免疫学试剂，可增强免疫反应。直接后果是与仅使用抗原相比，会产生更多的抗体和更长的免疫力，从而使疫苗所需的抗原剂量降至最低。特别是，基于DOTAP（1,2-油酰基--3-三甲基铵-丙烷）的阳离子脂质可诱导树突状细胞成熟，然后运输至引流淋巴结以激活抗原特异性T细胞。疫苗佐剂OZ生物科学VaxOZ佐剂是体内制造和质量控制的产品，仅用于研究目的。- AlumVax氢氧化物 / 磷酸盐，两个古典铝基佐剂- IFAVax， 不完全弗氏佐剂- CFAVax，完全弗氏佐剂- SqualVax，一个纳米油包水乳液-  CaLiVax-DOTAP， 对于脂质体介导的DNA或蛋白质疫苗基于阳离子脂质的组合物。