The ROS Detection Assay kit (Green Fluorescence) is a cell-based assay designed for measuring Reactive Oxygen Species (ROS) activity within a cell using the cell-permeable fluorogenic probe 2’, 7’-Dichlorodihydrofluorescein diacetate (DCF-DA).

Once DCF-DA has diffused into cells it is deacetylated by cellular esterases to a non-fluorescent compound and rapidly oxidized by ROS into DCF. DCF is highly fluorescent and can be detected by microscopy, titration in microplate and also cytometry (ex 485/em 535). The fluorescence intensity that results is proportional to the ROS levels within the cell.  

Main Features

• Measure ROS production in live cells

• Ideal for fluorescence microscopy, microplate titration and cytometry

• Compatible with adherent cells as well as suspension cells

Kit content

For up to 300 assays in 96-well plate

Tert-Butyl Hydrogen Peroxide (TBHP) 25 mM (50 µL)

Each ROS Kit provides sufficient reagents for approximately 300 measurements in microplate format and includes Tert-Butyl hydroperoxide (organic peroxide) solution provided as a positive control for ROS generation. 

 

Storage: Upon reception, store the DFC-DA tube (avoid multiple freeze/thawing cycles) and the 10X ROS Buffer at -20°C. Store the TBHP tube at 4°C.

 

 

Shipping Conditions: Shipped with ice pack

 

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