hLIF
| Product Name: | Recombinant Human LIF Protein |
| Description: | Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of pluripotent embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor (LIF) has a number of other functions including cholinergic neuron differentiation, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Human LIF has a molecular mass of 20 kDa containing 181 amino acid residues and is active on both human and mouse cells. Mouse LIF is about 1000 fold less active on human cells, than human LIF. |
| Available Sizes: | 10ug (10^6 units/ml), 100ug (10^7 units/ml) |
| Catalog Number: | 12-0002-10, 12-0002-100 |
| Source: | Escherichia Coli. |
| Purity: | Greater than 98%, as determined by SDS-PAGE |
| Endotoxin: | Less than 0.01 ng/μg cytokine as determined by the LAL assay |
| Storage: | This product is shipped at ambient temperature with cold pack. It is stable for up to 6 months from date of receipt when stored at 4 °C. Freeze-thaw should be avoided as it can result in loss of activity. |
| Formulation: | Human LIF is a 0.22 μm filtered sterile liquid in phosphate-buffered saline with 0.02% Tween-20 and 1% BSA. |
| Suggested Usage: | 1 ml of human LIF with the specific activity of 1x106 units/ml is sufficient to treat 1 L of embryonic stem cell culture media.1 ml of human LIF with the specific activity of 1x units/ml is sufficient to treat 10 L of embryonic stem cell culture media. |
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