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Lumiprobe点击化学染料 | cy3 cy5 cy7荧光染料

作者: 时间:2024-09-20 点击量:

Lumiprobe Sulfo-Cyanine7 NHS ester

Sulfo-Cyanine7 NHS ester是水溶近红外染料sulfo-Cyanine7,是胺类活性琥珀酸亚胺酯。

Sulfo-Cyanine7是Cy7®荧光团的的改良衍生物,量子产率提高20%,并具有更好的耐光性。该染料尤其适用于NIR成像应用。

近红外荧光染料利用了生物组织在特定的波长范围里的透明度。这种方法是无损伤性的,并允许在活体内对多种已标记的生物分子的分布进行跟踪。

Sulfo-Cyanine7 NHS ester可以很轻松的标记生物分子,如:蛋白质。标记的分子可以用于后续多种研究及药物开发相关的实验。

这种染料有很高的水溶解度,尤其适用于痕量蛋白质和易变性蛋白质的标记。需有机溶剂才能溶解的非磺化的Cyanine7 NHS ester。

Ayala-Orozco, C.; Liu, J.G.; Knight, M.W.; Wang, Y.; Day, J.K.; Nordlander, P.; Halas, N.J. Fluorescence Enhancement of Molecules Inside a Gold Nanomatryoshka. Nano Letters, 2014, 14(5), 2926-2933. doi: 10.1021/nl501027jBrand, C.; Abdel-Atti, D.; Zhang, Y.; Carlin, S.; Clardy, S.M.; Keliher, E.J.; Weber, W.A.; Lewis, J.S.; Reiner, T. In Vivo Imaging of GLP-1R with a Targeted Bimodal PET/Fluorescence Imaging Agent. Bioconjugate Chemistry, 2014, 25(7), 1323-1330. doi:10.1021/bc500178dGraen, T.M.D.; Hoefling, M.; Grubmüller, H. AMBER-DYES: Characterization of Charge Fluctuations and Force Field Parameterization of Fluorescent Dyes for Molecular Dynamics Simulations. Journal of Chemical Theory and Computation, 2014, 10(12), 5505-5512. doi: 10.1021/ct500869pLi, L.-L.; Xu, J.-H.; Qi, G.-B.; Zhao, X.; Yu, F.; Wang, H. Core-Shell Supramolecular Gelatin Nanoparticles for Adaptive and On-Demand Antibiotic Delivery. ACS Nano, 2014, 8(5), 4975-4983. doi: 10.1021/nn501040hViehweger, K.; Barbaro, L.; García, K.P.; Joshi, T.; Geipel, G.; Steinbach, J.; Stephan, H.; Spiccia, L.; Graham, B. EGF Receptor-Targeting Peptide Conjugate Incorporating a Near-IR Fluorescent Dye and a Novel 1,4,7-Triazacyclononane-Based 64Cu(II) Chelator Assembled via Click Chemistry. Bioconjugate Chemistry, 2014, 25(5), 1011-1022. doi: 10.1021/bc5001388Zhou, Z.; Wu, X.; Kresak, A.; Griswold, M.; Lu, Z.-R. Peptide targeted tripod macrocyclic Gd(III) chelates for cancer molecular MRI.Biomaterials, 2013, 34(31), 7683-7693. doi: 10.1016/j.biomaterials.2013.06.057Zou, C.; Loka, R.S.; Zhang, Y.; Cairo, C.W. Glycoform Remodeling Generates a Synthetic T Cell Phenotype. Bioconjugate Chemistry, 2013, 24(6), 907-914. doi: 10.1021/bc300599wNakamura, T.; Sugihara, F.; Matsushita, H.; Yoshioka, Y.; Mizukami, S.; Kikuchi, K. Mesoporous silica nanoparticles for 19F magnetic resonance imaging, fluorescence imaging, and drug delivery. Chemical Science, 2015. doi: 10.1039/c4sc03549fAldeek, F.; Hawkins, D.; Palomo, V.; Safi, M.; Palui, G.; Dawson, P.E.; Alabugin, I.V.; Mattoussi, H. UV and Sunlight Driven Photoligation of Quantum Dots: Understanding the Photochemical Transformation of the Ligands. Journal of the American Chemical Society, 2015. doi: 10.1021/ja512802xHsueh, P.-Y.; Edman, M.C.; Sun, G.; Shi, P.; Xu, S.; Lin, Y.-a.; Cui, H.; Hamm-Alvarez, S.F.; MacKay, J.A. Tear-mediated delivery of nanoparticles through transcytosis of the lacrimal gland. Journal of Controlled Release, 2015. doi: 10.1016/j.jconrel.2014.12.017Wen, A.M.; Infusino, M.; De Luca, A.; Kernan, D.L.; Czapar, A.E.; Strangi, G.; Steinmetz, N.F. Interface of Physics and Biology: Engineering Virus-Based Nanoparticles for Biophotonics. Bioconjugate Chemistry, 2015, 26(1), 51–62. doi: 10.1021/bc500524f

Cy3/5/7 NHS ester和带NH2多肽抗体连接实验方法及步骤

Cy3 NHS ester, Cyanine5 NHS ester和Cyanine7 NHS ester可以标记含有amine的多肽,抗体,蛋白,DNA和寡核苷酸及其他小分子。在反应条件下NHS可以和NH2发生反应,从而连接到一起。

(一):Cyanine5 NHS ester和多克隆抗体的连接实验方法和步骤

【准备材料】:

Cy3 NHS ester purchased from xi an ruixibio

anti-GST多克隆抗体purchased from Abcam

【实验步骤】:

1.      在1 L 0.15 M NaCl 溶液中透析anti-GST抗体(浓度为 0.5 mL at3 mg/mL),常温透析4小时。

2.      在4°C用新鲜的1 L 0.15 M NaCl 溶液再透析过夜。

3.      第二天用1 L 0.1 M NaHCO3 (pH 8.3)透析4小时。

4.      用0.22 μm注射器过滤头过滤抗体溶液。

5.      用0.1 M NaHCO3 稀释少量的抗体,在280nm处测其紫外吸收值计算标记抗体的总量 (IgG antibody摩尔吸光系数170 000 M-1 cm-1 at 280 nm).

6.      用DMSO配置Cy3 NHS (MW 616.19)溶液浓度为10 mg/mL;计算所需体积以得到想要的Cy3NHS 和抗体的比值(例如20:1),然后慢慢将其加入到抗体溶液中,同时在暗处常温缓慢搅拌45分钟。

7.      用1 L 0.15 M NaCl 溶液常温避光透析4小时除去未标记上的Cy5。

8.      在4°C用新鲜的1 L 0.15 M NaCl 溶液再避光透析过夜。

9.      用1 L of 0.01 M PBS/ 0.01% 叠氮化钠溶液常温避光透析4小时, 在4°C常温避光再次透析过夜。

10.  用0.22 μm注射器过滤头过滤抗体溶液。

11.  用0.01 M PBS/0.01 % 叠氮化钠整数倍稀释标记抗体溶液,测量280nm(蛋白)和552nm(Cy)处的紫外可见吸光度。

12.  产品冷冻干燥成粉末或在0.01 M PBS/ 0.01% 叠氮化钠溶液中,-20℃避光储存。

F/P计算:

Cy3在552nm摩尔吸光系数为150 000M-1 cm-1;此蛋白在280nm的摩尔吸光系数为170 000 M-1 cm-1;

不同蛋白的摩尔吸光系数不一样;.Cy3染料本身在280 nm 的吸收是552nm处的8%。按以下公式计算F/P

值。

[Cy3] = A552/150000

[antibody] = {A280 (0.08×A552)}/170000

F/P final= [Cy3]/[antibody]= {1.13×A552}/{A280 (0.08×A552)}

(二):Cyanine5 NHS ester和标记 (D-ser2)-leucineenkephalin的连接实验方法和步骤

【准备材料】:

Cy5 NHS ester purchased from xi an ruixibio

(D-ser2)-leucineenkephalin purchased fromsigma

【实验步骤】:

1.      Cy5 NHS 1.0 mg 溶解于400 μL DMSO后,加入到1mL 的玻璃瓶中盛有(D-ser2)-leucine-enkephalinacetate(YSGFLT, 0.75 mg) 的400 μL DMSO溶液。(Dye 和peptide 投料比是 1:1)

2.      然后加入15 μL三乙胺,常温避光搅拌反应混合物过夜。

3.      用HPLC 纯化蛋白,使用蛋白C18柱子(25 cm×10 mm),每次上样注入2×400 μL,30分钟梯度洗脱从0.1% TFA水溶液到MeCN∶H2O(0.1% TFA)=70∶30,流速4mL /min。(对不同的蛋白选择不同的合适HPLC梯度流动相)

4.      收集适当的色带峰,标记多肽的保留时间比未标记的多肽长。

5.      产品冷冻干燥成粉末或在水溶液中,-20℃避光储存;必要时可用质谱表征。

6.      CyDye标记的蛋白稳定性取决于蛋白本身。例如标记的IgG在4 ℃可避光保存2月;更长期的保存需加入等体积的甘油-20 ℃避光保存.

F/P计算:

Cy5 在650 nm 的摩尔吸光系数为250 000 M-1 cm-1 ,所用蛋白在280 nm处的摩尔吸光系数为 170

000 M-1 cm-1;Cy5在280nm处的吸收是650nm处的5%。按以下公式计算F/P值。

[Cy5 dye] = (A650)/250 000

[peptide] =[A280 -(0.05×A650)]/170 000

F/P final= [dye]/[peptide]= {0.68×A650}/{A280 (0.05×A650)}

上海金畔生物科技有限公司产品种类:

Cyanine3 alkyne

Cyanine3 azide

Cyanine3 carboxylic acid  1144107-76-5

Cyanine3 hydrazide

Cyanine3 maleimide

Cyanine3 NHS ester   1032678-38-8

Cyanine3.5 azide

Cyanine3.5 carboxylic acid  1144107-79-8

Cyanine3.5 NHS ester

Cyanine5 alkyne

Cyanine5 amine

Cyanine5 azide

Cyanine5 Boc-hydrazide

Cyanine5 carboxylic acid

Cyanine5 hydrazide

Cyanine5 maleimide

Cyanine5 NHS ester  1032678-42-4

Cyanine5.5 alkyne

Cyanine5.5 azide

Cyanine5.5 carboxylic acid  1144107-80-1

Cyanine5.5 maleimide

Cyanine5.5 NHS ester

Cyanine7 amine   1650635-41-8

Cyanine7 azide

Cyanine7 carboxylic acid

Cyanine7 maleimide

Cyanine7 NHS ester

Cyanine7.5 amine

Cyanine7.5 azide

Cyanine7.5 carboxylic acid   1686147-68-1

Cyanine7.5 maleimide

Cyanine7.5 NHS ester

Sulfo-Cyanine 5 NHS ester

Sulfo-Cyanine3 azide

Sulfo-Cyanine3 carboxylic acid

Sulfo-Cyanine3 maleimide

Sulfo-Cyanine3 NHS ester

Sulfo-Cyanine5 alkyne

Sulfo-Cyanine5 azide

Sulfo-Cyanine5 carboxylic acid   1121756-16-8

Sulfo-Cyanine5 maleimide

Sulfo-Cyanine7 azide

Sulfo-Cyanine7 carboxylic acid

Sulfo-Cyanine7 NHS ester

Storage conditions:Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

上海金畔生物科技有限公司(www.jinpanbio.cn)提供生命科学研究领域系列产品,包括生化试剂、色谱标准品和实验仪器耗材。主营Lumiprobe Cy系列活性荧光染料;WAKO日本和光纯药、修饰性PEG(Laysan bio、NANOCS、Avanti等品牌PEG以及定制合成修饰性聚乙二醇、单分散小分量PEG);Sigma、Amresco、TCI、MP bio生化试剂;日本关东化学Kanto试剂、日本三菱、日本柴田科学SIBATA;Megazyme食品分析检测试剂盒、日本共立理化学;Harlan饲料、Bio-Serv、日本CLEA Japan品牌的动物饲料;Oxoid、Nissui日水、日本荣研、BD difco、Himedia品牌微生物培养基;免疫试剂包括:Bethyl抗体;Biolegend流式抗体、Abcam、CST、Santa Cruz抗体;Roche、TOYOBO、NEB品牌的酶;中检所、TRC、药典USP、EP、Reagecon标准品;耗材和仪器包括Whatman、日本Advantec滤膜、Millipore品牌的各种滤膜、滤器和柱子填料等、Hampton蛋白结晶试剂耗材、小鼠软管灌胃针、动物毛发记号笔、Labnet、Wheaton瓶子、Bio-Rad伯乐、康宁Corning、Axygen、Falcon 、Eppendorf、Nunc、Nalgene、Nest品牌的培养皿、培养板、离心机、离心管、移液枪及枪头等实验室常用仪器耗材。

Lumiprobe 羧基类、羰基类荧光染料

Lumiprobe Corporation是美国一家高品质生物技术公司,专业提供分子生物学研究用的活性荧光染料。从2006年开始,公司生产并销售生命科学研究和诊断学应用的优质化学药品。产品主要有:活性染料(Reactive Dye)和SYBR Green I 染料,用于寡核苷酸合成的亚磷酰胺,点击化学用染料和其它试剂。

产品主要应用:点击化学(Click Chemistry)、蛋白质组学研究中的双向荧光差异凝胶电泳(2D DIGE)和实时荧光定量PCR(Realtime PCR)。氨基类染料是包含自由氨基的活性染料,染料可与活化羧酸衍生物和其他亲电子的试剂结合。比如:氨基与EDC-活化的羧基结合。上海金畔生物科技有限公司是Lumiprobe一级代理,可提供Lumiprobe产品和具有竞争力的价格,包括:羧酸类染料、羰基类染料,标记分子氨基活性染料,标记分子巯基活性染料,动物活体成像用染料,蛋白质、多肽、核酸等生物分子标记染料等。羰基类染料羰基活性染料用于标记生物分子中的醛和酮。这类染料可与生物分子的羰基温和地发生反应。比如对氧敏感的蛋白,可被高碘酸盐氧化作用激活的糖蛋白和带有羰基的寡核苷酸。

Lumiprobe 磺酸基-Cy5-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine5 NHS ester

水溶Cyanine 5琥珀酰亚胺酯(succinimidyl ester(SE)),类似于Cy5® NHS ester,不需要有机溶剂即在水相中即可对各种带氨基分子进行标记。因此该产品尤其适用于对那些在有机溶剂中易变性的蛋白和低溶解度蛋白的标记。Sulfo-Cyanine 5是Cy5®的衍生物,与很多仪器如读板机(plate readers),显微镜和成像设备等相兼容。是最受欢迎的荧光染料之一。该染料是高度亲水的、水溶的。非磺化的衍生物:Cyanine 5 NHS ester。可以在所有的应用中替代Cy5®,Alexa Fluor 647和DyLight 649。

Lumiprobe 磺酸基-Cy5-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine5 NHS ester   一般特性

储存条件:Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

上海金畔生物科技有限公司还可以提供其他相关产品:

Cyanine3 alkyne

Cy3 alkyne

Cyanine3 azide

Cy3 azide

Cy3 N3

Cyanine3 carboxylic acid  1144107-76-5

Cy3 carboxylic acid

Cyanine3 hydrazide

Cyanine3 maleimide

Cy3 maleimide

Cyanine3 NHS ester   1032678-38-8

Cy3 NHS ester

Cyanine3.5 azide

Cyanine3.5 carboxylic acid  1144107-79-8

Cyanine3.5 NHS ester

Cy3.5 NHS ester

Cyanine5 alkyne

Cyanine5 amine

Cy5 amine

Cy5 NH2

Cyanine5 azide

Cy5 N3

Cyanine5 Boc-hydrazide

Cyanine5 carboxylic acid

Cyanine5 hydrazide

Cyanine5 maleimide

Cy5 maleimide

Cyanine5 NHS ester  1032678-42-4

Cy5 NHSester

Cyanine5.5 alkyne

Cyanine5.5 azide

Cyanine5.5 carboxylic acid  1144107-80-1

Cyanine5.5 maleimide

Cyanine5.5 NHS ester

Cyanine7 amine   1650635-41-8

Cy7 amine

Cy7 NH2

Cyanine7 azide

Cy7 azide

Cyanine7 carboxylic acid

Cyanine7 maleimide

Cy7 maleimide

Cyanine7 NHS ester

Cy7 NHS ester

Cyanine7.5 amine

Cyanine7.5 azide

Cyanine7.5 carboxylic acid   1686147-68-1

Cyanine7.5 maleimide

Cyanine7.5 NHS ester

Sulfo-Cyanine 5 NHS ester

Sulfo-Cy 5 NHS ester

Sulfo-Cyanine3 azide

Sulfo-Cy3 azide

Sulfo-Cyanine3 carboxylic acid

Sulfo-Cyanine3 maleimide

Sulfo-Cy3 maleimide

Sulfo-Cyanine3 NHS ester

Sulfo-Cy3 NHS ester

Sulfo-Cyanine5 alkyne

Sulfo-Cyanine5 azide

Sulfo-Cy5 azide

Sulfo-Cyanine5 carboxylic acid   1121756-16-8

Sulfo-Cyanine5 maleimide

Sulfo-Cy5 maleimide

Sulfo-Cyanine7 azide

Sulfo-Cyanine7 carboxylic acid

Sulfo-Cyanine7 NHS ester

Sulfo-Cy7 NHS ester

Lumiprobe 磺酸基-Cy3 叠氮化物 Sulfo-Cyanine3 azide

上海金畔生物科技有限公司作为Lumiprobe中国正规签约授权代理商,Lumiprobe上海金畔生物官网:http://lumiprobe.jinpanbio.com/。可提供Lumiprobe产品,货期10工作日左右,满足您的各种科研实验需求。Lumiprobe产品包括:羧酸类染料、羰基类染料,标记分子氨基活性染料,标记分子巯基活性染料,动物活体成像用染料,蛋白质、多肽、核酸等生物分子标记染料、点击化学等。渠道正规(市场上部分是分装,质量不能保证),质量保证,价格优惠。

服务热线:18301939375   QQ:3258089810    3259632176

Sulfo-Cyanine3 azide is a water-soluble dye azide for Click Chemistry. Absorbance and emission of the dye are identical to Cy3® fluorophore.

Brightness and photostability of fluorophore, together with high water solubility, makes this reagent an ideal choice for Click chemistry labeling of various molecules in aqueous phase.

Click reaction can be carried out in pure water without need to use organic co-solvent. The reagent is designed for the labeling of sensitive molecules such as proteins, and even intact biological objects.Lumiprobe 磺酸基-Cy3 叠氮化物 Sulfo-Cyanine3 azide

General properties

Storage conditions:Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.Spectral properties Lumiprobe 炔烃染料 Dye alkynes

Lumiprobe Corporation是美国一家高品质生物技术公司,专业提供分子生物学研究用的活性荧光染料。从2006年开始,公司生产并销售生命科学研究和诊断学应用的优质化学药品。产品主要有:活性染料(Reactive Dye)和SYBR Green I 染料,用于寡核苷酸合成的亚磷酰胺,点击化学用染料和其它试剂。 产品主要应用:点击化学(Click Chemistry)、蛋白质组学研究中的双向荧光差异凝胶电泳(2D DIGE)和实时荧光定量PCR(Realtime PCR)。

上海金畔生物科技有限公司作为Lumiprobe中国正规签约授权代理商,Lumiprobe上海金畔生物官网:http://lumiprobe.jinpanbio.com/。可提供Lumiprobe产品,产品货期10个工作日左右,满足您的各种科研实验需求。Lumiprobe产品包括:羧酸类染料、羰基类染料,标记分子氨基活性染料,标记分子巯基活性染料,动物活体成像用染料,蛋白质、多肽、核酸等生物分子标记染料、点击化学等。渠道正规,质量保证,价格优惠。

Lumiprobe 炔烃染料 Dye alkynes

炔烃 Dye alkynes

1-乙炔基芘1-Ethynyl pyrene

Pyrene is polyaromatic hydrocarbon containing four fused rings. This reagent contains terminal alkyne group for Click Chemistry and other reactions, such as Sonogashira coupling.

3-乙炔基芘3-Ethynyl perylene

Alkyne derivative of ultra-bright and photostable perylene fluorophore.

Cy3 炔烃Cyanine3 alkyne

Cyanine3 alkyne is a fluorescent dye alkyne for Click Chemistry.

Cy5 炔烃Cyanine5 alkyne

Cyanine5 alkyne is an alkyne dye for Click Chemistry.

Cy5.5 炔烃Cyanine5.5 alkyne

Cyanine5.5 alkyne is a NIR (near infrared) dye azide for both copper-catalyzed, and copper-free Click Chemistry.

FAM 炔烃,5-异构体FAM alkyne, 5-isomer

FAM alkyne, 5-isomer, alkyne for Click Chemistry

磺酸基-Cy5 炔烃 Sulfo-Cyanine5 alkyne

Sulfo-Cyanine5 is a water-soluble Cyanine5 dye alkyne for Click chemistry.

Lumiprobe 炔烃染料 Dye alkynesLumiprobe Corporation是美国一家高品质生物技术公司,专业提供分子生物学研究用的活性荧光染料。从2006年开始,公司生产并销售生命科学研究和诊断学应用的优质化学药品。产品主要有:活性染料(Reactive Dye)和SYBR Green I 染料,用于寡核苷酸合成的亚磷酰胺,点击化学用染料和其它试剂。 产品主要应用:点击化学(Click Chemistry)、蛋白质组学研究中的双向荧光差异凝胶电泳(2D DIGE)和实时荧光定量PCR(Realtime PCR)。

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BDP FL 叠氮化物 BDP FL azide

BDP FL is a bright and photostable borondipyrromethene dye for fluorescein channel. This is a dye azide for Click Chemistry.

香豆素343 叠氮化物 Coumarin 343 azide

Fluorescent dye azide for Coumarin 343 labeling via Click Chemistry.

Cy3 叠氮化物 Cyanine3 azide

Azide derivative of Cyanine3 fluorescent dye for Click Chemistry.

Cy3.5 叠氮化物 Cyanine3.5 azide

Cyanine3.5 azide for biomolecule labeling through Click Chemistry.

Cy5 叠氮化物 Cyanine5 azide

Cyanine5 azide is a reagent for Click Chemistry labeling with Cyanine5, a popular fluorescent dye.

Cy5.5 叠氮化物 Cyanine5.5 azide

Far red / near infrared fluorescent Cyanine5.5 dye derivative for Click Chemistry.

Cy7 叠氮化物 Cyanine7 azide

Near-infrared fluorescent dye for Click Chemistry.

Cy7.5 叠氮化物 Cyanine7.5 azide

Long-wavelength NIR fluorescent dye azide for Click chemistry.

5-FAM 叠氮化物 FAM azide, 5-isomer

FAM (fluorescein) azide for Click chemistry, pure 5-isomer.

6-FAM 叠氮化物 FAM azide, 6-isomer

FAM azide, 6-isomer is a commonly used fluorescent dye azide for Click Chemistry.

苝叠氮化物 PEP azide

PEP (phenylethynylpyrene), polyaromatic hydrocarbon fluorescent dye for Click-Chemistry labeling.

芘叠氮化物 1 Pyrene azide 1

Reagent for fluorescent labeling with pyrene polycyclic aromatic hydrocarbon via Click Chemistry.

芘叠氮化物 2 Pyrene azide 2

Pyrene azide with longer linker, for Click Chemistry.

芘叠氮化物 3 Pyrene azide 3

Pyrene azide with even longer linker for Click Chemistry.

5-ROX 叠氮化物 ROX azide, 5- isomer

ROX (aka rhodamine X or rhodamine 101) azide for biomolecule labeling through Click Chemistry.

磺酸基-Cy3 叠氮化物 Sulfo-Cyanine3 azide

Water-soluble sulfo-Cyanine3 dye azide for Click Chemistry.

磺酸基-Cy5 叠氮化物 Sulfo-Cyanine5 azide

Water soluble sulfo-Cyanine5 fluorescent dye azide for Click Chemistry.

磺酸基-Cy7 叠氮化物 Sulfo-Cyanine7 azide

Sulfo-Cyanine7 NHS ester is a water soluble, near infrared, amine-reactive dye.

5-四甲基罗单明 叠氮化物 TAMRA azide, 5- isomer

TAMRA fluorescent dye azide for Click chemistry.

Lumiprobe 羧酸染料 Carboxylic acid

Lumiprobe Corporation是美国一家高品质生物技术公司,专业提供分子生物学研究用的活性荧光染料。从2006年开始,公司生产并销售生命科学研究和诊断学应用的优质化学药品。产品主要有:活性染料(Reactive Dye)和SYBR Green I 染料,用于寡核苷酸合成的亚磷酰胺,点击化学用染料和其它试剂。 产品主要应用:点击化学(Click Chemistry)、蛋白质组学研究中的双向荧光差异凝胶电泳(2D DIGE)和实时荧光定量PCR(Realtime PCR)。

羧酸染料 Carboxylic acid

Cy3羧酸 Cyanine3 carboxylic acid

Free Cyanine3 carboxylic acid.

Cy3.5羧酸 Cyanine3.5 carboxylic acid

Unactivated monofunctional Cy3.5 carboxylic acid.

Cy5羧酸 Cyanine5 carboxylic acid

Cyanine5 carboxylic acid, unactivated dye.

Cy5.5羧酸 Cyanine5.5 carboxylic acid

Cy5.5 unactivated free acid cyanine dye.

Cy7羧酸 Cyanine7 carboxylic acid

Non-reactive, near-infrared fluorescent dye.

Cy7.5羧酸 Cyanine7.5 carboxylic acid

Cyanine7.5 unactivated NIR cyanine dye carboxylic acid.

磺酸基-Cy3羧酸 Sulfo-Cyanine3 carboxylic acid

Sulfo-Cyanine3 free unactivated monofunctional carboxylic acid, water-soluble dye.

磺酸基-Cy5羧酸 Sulfo-Cyanine5 carboxylic acid

Water-soluble Cyanine5 carboxylic acid, non-activated fluorescent dye.

磺酸基-Cy7羧酸 Sulfo-Cyanine7 carboxylic acid

Sulfo-Cy7 carboxylic acid is a non-reactive fluorescent dye which can be used as a NIR (near infrared) marker.

Lumiprobe 羧酸染料 Carboxylic acid

Lumiprobe 氨基染料 Amino dyes

       Lumiprobe Corporation是美国一家高品质生物技术公司,专业提供分子生物学研究用的活性荧光染料。从2006年开始,公司生产并销售生命科学研究和诊断学应用的优质化学药品。产品主要有:活性染料(Reactive Dye)和SYBR Green I 染料,用于寡核苷酸合成的亚磷酰胺,点击化学用染料和其它试剂。 产品主要应用:点击化学(Click Chemistry)、蛋白质组学研究中的双向荧光差异凝胶电泳(2D DIGE)和实时荧光定量PCR(Realtime PCR)。

氨基染料 Amino dyes

炔烃CPG修饰剂 500Alkyne CPG modifier 500

Solid support for the synthesis of 3 -modified oligonucleotides with alkyne group for Click Chemistry.

Cy3胺 Cyanine3 amine

Cyanine3 is an fluorophore with free amino group (amino-dye). It can be conjugated with NHS esters, carboxy groups (after activation), and epoxides.

Cy5胺 Cyanine5 amine

Cyanine5 amine functionalized dye.

Cy5.5胺酯 Cyanine5.5 amine

Amino derivative of Cyanine5.5, a far red emitting fluorescent cyanine dye.

Cy7胺酯 Cyanine7 amine

Cy7 amine is a NIR dye with free amine group.

Cy7.5胺酯 Cyanine7.5 amine

Near infrared dye Cyanine7.5 with free amino group.

磺酸基-Cy7-胺 Sulfo-Cyanine7 amine

Water-soluble NIR dye amine, sulfonated derivative of Cyanine7 dye, for the modification via enzymatical transamination.

Lumiprobe 氨基染料 Amino dyes

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