Anti-GFP antibody

cat.# AB011

Rabbit polyclonal antibody against EGFP, BrUSLEE, SLIM, TagCFP, TagGFP, TagGFP2, TagYFP, PS-CFP2, AceGFP1, Case12 and HyPer

PRODUCT	CAT.#	SIZE	PRICE	DATA SHEETS
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Anti-GFPantibody	AB011	100 μg	€ 220

SpecificityThe antibody has been selected to recognize both denatured and native EGFP, BrUSLEE, SLIM, TagCFP, TagGFP, TagGFP2, TagYFP, PS-CFP2, AceGFP1, Case12 and HyPer.

ImmunogenFull-length recombinant non-denatured TagGFP2 and EGFP.

ReconstitutionReconstitute with sterile water or 50% glycerol.

StorageLyophilized samples are stable for twelve months from date of receipt when stored at -20°C. The presence of silica gel drier is advisable.Reconstituted with sterile water, antibody can be stored at +2 – +8°C for three months without detectable loss of activity.Reconstituted with 50% glycerol, antibody can be stored at -20°C in a manual defrost freezer for six months without detectable loss of activity. Aliquot antibody upon reconstitution. Avoid repeated freeze / thaw cycles.

Use

• Western blot
• Immunoblotting
• ICC
• ELISA

Evrogen的基因编码的光诱导细胞周期抑制剂ArrestRed-细胞周期进程的可逆抑制-激活由绿色光照射-直接表达和在细胞核容易可视化-无外源化学品化合物所需-推荐用于细胞分裂的瞬时堵塞在体外和体内 ArrestRed（科学名称为H2B-tKR）是一种新型的光遗传学工具，可用于研究特定细胞群在发育，再生和癌变中的作用。这是一种嵌合蛋白，由串联编码的与组蛋白H2B蛋白融合的基因编码的光敏剂KillerRed组成 [Serebrovskaya 等。，2011]。ArrestRed在哺乳动物细胞中的表达可导致正确的染色质标记，并且不会干扰细胞分裂。强烈的绿光照亮表达ArrestRed的细胞会导致细胞增殖受阻。ArrestRed激活的作用持续约24小时，此后约90％的ArrestRed表达细胞恢复分裂。重复的光照可使细胞保持非分裂状态更长的时间。ArrestRed对细胞周期进程的抑制作用归因于ArrestRed活化后产生的活性氧（ROS）[Bulina 等。，2006]。光诱导产生的ROS会导致基因组DNA的大量破坏和修复机制的激活。反过来，它导致细胞周期检查点激活和细胞周期停滞。成功修复DNA后，相间细胞恢复正常增殖。