PDK1 (Phospho-Ser241) AntibodyPDF
Catalog Number:
E011005-2Amount:
100μg/100μlForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearImmunogen:
The antiserum was produced against synthesized phosphopeptide derived from human PDK1 around the phosphorylation site of serine 241 (A-N-SP-F-V).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
PDK1 (phospho-Ser241) antibody detects endogenous levels of PDK1 only when phosphorylated at serine 241.Reactivity:
Human, Mouse, RatApplications:
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100-200Swiss-Prot No.:
O15530References:
Scheid MP,et al. (2005)Mol Cell Biol; 25(6): 2347-63Chen H, et al. (2001) Biochemistry; 40(39): 11851-9Sato S,et al. (2002) J Biol Chem; 277(42): 39360-7Lim MA, et al.(2003)Proc Natl Acad Sci U S A; 100(24): 14006-11P-Peptide - +Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using PDK1 (phospho-Ser241) antibody (E011005).
Western blot analysis of extracts from MDA-MB-435 cells using PDK1 (phospho-Ser241) antibody (E011005).
Price:
225$Research Area:
Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells