PurKine™ Protein A Purification system is designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Recombinant protein A is produced in E. coli and functions essentially the same as native Protein A from Staphylococcus aureus. The Protein A molecule contains four high-affinity binding sites capable of interacting with the Fc region from IgG of several species. Protein A is generally preferred for affinity purification of rabbit, Guinea pig, dog and cat IgG. The portfolio allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ Protein A Purification Resin is also available as prepacked spin column and kit formats.

PurKine™ Protein A Resin has been immobilized for use in IgG purification applications in gravity column procedures at a variety of scales. The Resin consists of 90?m beads of cross-linked 4% agarose, to which Recombinant protein A has been coupled. Tests confirm that performance equals or exceeds popular Recombinant protein A resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.