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| Product Code | pro0001 |
|---|---|
| Uniprot ID | P22735 |
| Name | Transglutaminase-1 (TGase-1) |
| Appearance | White lyophilized solid |
|---|---|
| Constituents | The Transglutaminase is lyophilized from 50 mM Tris-HCl pH 8.0, 10 mM Glutathion. |
| Conc. / Activity | 8201 U/mg |
| Storage | Store working aliquots at a minimum of -20°CAvoid repeated freezing and thawingDelivery at ambient temperature is possible |
| Description | His6-rhTG1 is based on the TGM1-allele from I.M.A.G.E.-clone IRAKp961M1628 isolated from human skin squamous cell carcinoma. It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MHHHHHHMDGPR. His6-rhTG1 is purified by IMAC to more than 90 % purity. |
| Produced in | Insect cells |
| Species | Human |
| Form | Recombinant |
| Gene ID | 7051 |
| Labelling | His-Tag |
| Preparation method | Add 10 mM Ca2+ to activate His6-rhTG1. |
| Molecular weight | 90 kDa |
| Principle | His6-rhTG1 catalyzes acyl transfer reactions from glutamin residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG1 may also be used for immunoprecipitation. |
| Purity | > 90 % (visually by SDS-PAGE) |
| Recommendations | Add 340 µl of distilled water to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be cooled on ice for short term storage. Protein concentration (determined on reconstituted rhTG1): 0.46 mg/ml |
| Product type | Proteins |
| Applications | Immunoprecipitation, Functional Assay |
