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Product Code | pab72983 |
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Immunogen | Recombinant protein fragment containing a sequence corresponding to a region within amino acids 9 and 323 of GAPDH. |
Target Specificity | Reacts with human GAPDH. Cross reacts with drosophila, mouse and rat protein. |
Name | GAPDH |
Uniprot ID | P04406 |
Product type | Primary antibodies, Loading controls |
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Clonality | Polyclonal antibody |
Produced in | Rabbit |
Species | Human, Mouse, Rat, Drosophila melanogaster |
Labelling | None |
Appearance | Liquid |
Form | Antigen affinity purified |
Constituents | PBS, pH 7, 1% BSA, glycerol 20% |
Preservatives | Thiomersal 0.01% |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Applications | Western Blot, IHC - Paraffin sections, Immunofluorescence, Immunocytochemistry |
Working dilutions | Optimal dilution should be determined by the end user.The following are guidelines only :ICC (1:100 - 1:200) IF (1:100 - 1:200)IHC-P (10 µg/ml)WB (1:5000 - 1:20000) |
- (pab72983)Anti-GAPDH antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 µg/ml.">
- (pab72983)Anti-GAPDH antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 µg/ml.">
- (pab72983)Anti-GAPDH antibody IF of paraformaldehyde-fixed A549. GAPDH antibody at 1:200 dilution.">
- (pab72983)Anti-GAPDH antibody WB (30 ug) of whole cell lysate. A: 293T. B: A431. C: H1299. D: Hela. 10% SDS PAGE. GAPDH antibody at 1:5000 dilution">
- (pab72983)Anti-GAPDH antibody WB (50 ug) of whole cell lysate. A: Mouse Brain. 10% SDS PAGE. GAPDH Antibody at 1:10000 dilution">