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Product Code | mab90004-P |
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Clone | E10 / V4RR |
Immunogen | GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH |
Target Specificity | Reacts with V5-tagged fusion proteins. |
Name | V5-Tag |
Product type | Primary antibodies, Tags |
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Clonality | Monoclonal antibody |
Isotype | IgG1 |
Produced in | Mouse |
Species | Human, Mouse, Rat, Monkey, Chicken |
Labelling | None |
Appearance | Liquid |
Form | Purified |
Constituents | PBS 10mM, (pH 7.2) |
Preservatives | NaN3 0.05% |
Storage | Short term storage at +4°C (add 0.1% NaN3).For extended periods store in aliquots at -20°C.Antibodies are guaranteed for 6 month from date of receipt. |
Applications | Western Blot, Immunofluorescence, Immunocytochemistry, ELISA, Immunoprecipitation |
Working dilutions | Optimal dilution should be determined by the end user. The following are guidelines only :WB (1:1000 - 1:3000)ELISA (1:100 - 1:2000)ICC/IF(1:500 - 1:2000)IP (1:10 - 1:500) |
- (mab90004-P)Anti-V5-Tag antibody WB staining of HEK293 cells (1) untransfected or (2)transfected with V5-tagged protein vector.">
- (mab90004-P)Anti-V5-Tag antibody WB staining of 15 µg of 293T cell lysates, from cells transfected with a V5-Wnt3a or V5-Vstm2 construct for the indicated number of hours, per well.">