CLIA Kit for Albumin (ALB)

CCB028Mu

96T

Competitive CLIA Kit

CLIA

Mouse

CLIA Kits

The microtiter plate provided in this kit has been pre-coated with a monoclonal antigen. A competitive inhibition reaction is launched between biotin labeled Albumin (ALB) and unlabeled Albumin (ALB) (Standards or samples) with the pre-coated secondary antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Albumin (ALB) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Albumin (ALB) level in the sample or standard.This assay has high sensitivity and excellent specificity for detection of Albumin (ALB). No significant cross-reactivity or interference between Albumin (ALB) and analogues was observed.

Detection Range: 781.25-200000ng/mL The standard curve concentrations used for the CLIA?s were 200000ng/mL, 50000ng/mL, 12500ng/mL, 3125ng/mL, 781.25ng/mL

Sensitivity: The minimum detectable dose of this kit is typically less than277ng/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 2.5hours

Cloud-Clone Corp.

CLIA Kits