CLIA Kit for Adrenocorticotropic Hormone (ACTH)

CCA836Ra

96T

Competitive CLIA Kit

CLIA

Rat

CLIA Kits

The microtiter plate provided in this kit has been pre-coated with a monoclonal antigen. A competitive inhibition reaction is launched between biotin labeled Adrenocorticotropic Hormone (ACTH) and unlabeled Adrenocorticotropic Hormone (ACTH) (Standards or samples) with the pre-coated secondary antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Adrenocorticotropic Hormone (ACTH) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Adrenocorticotropic Hormone (ACTH) level in the sample or standard.This assay has high sensitivity and excellent specificity for detection of Adrenocorticotropic Hormone (ACTH). No significant cross-reactivity or interference between Adrenocorticotropic Hormone (ACTH) and analogues was observed.

Detection Range: 11.72-3000pg/mL The standard curve concentrations used for the CLIA?s were 3000pg/mL, 750pg/mL, 187.5pg/mL, 46.88pg/mL, 11.72pg/mL

Sensitivity: The minimum detectable dose of this kit is typically less than4.22pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Length: 2.5hours

Cloud-Clone Corp.

CLIA Kits