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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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商品描述
3T3 cells
Product Code:
EXA-X1005
Pack Size:
100 µl
Form:
Control Lysate
Shipping Temp:
Room Temperature
Storage Temp:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Type:
Lysate
Description:
Total cell Iysates are useful as both positive and negative controls in immunoblotting. A431, a human epidermoid carcinoma cell line, and the EGF-stimulated A431 Iysates are used as negative and positive controls,respectively, when studying the phosphorylation cascade initiated by ligand binding to receptor tyrosine kinases. Both A431 and normal human fibroblasts are valuable positive controls for antibodies to proteins expressed in thesecells. The mouse fibroblast cell line 3T3 and its RSV-transformed counterpart serve as negative and positive controls, respectively, for antibodies to proteins that are phosphorylated as a result of transformation by RSV (Rous sarcomavirus). Madin-Darby bovine kidney (MDBK) cell Iysate displays proteins expressed in bovine kidney and PC12 (rat phaeochromocytoma) cell Iysate can be expected to mirror the protein expression of cells and tissues ofneuroectodermal origin. Likewise, Jurkat cells express the proteins unique to human T-cells. Rat brain is a widely used positive control for those proteins that are exclusively expressed in brain. At right is an example of the use of positiveand negative control Iysates in western blotting. The blot of 3T3 cell Iysate (lane 1) and RSV-transformed 3T3 (lane 2) wasprobed with anti-phosphotyrosine peroxidase conjugate to reveal those proteins that are tyrosine-phosphorylated in response to transformation by
Supplier:
Nordic MuBio
Product Category:
Cell Fractions & Lysates > Cell line Lysate
Applications: Suggested use: A volume of 10-25ul is suggested for immunobloting.Product Form: UnconjugatedFormulation: Provided as solution in Laemmli electrophoresis sample buffer. Samples need to be boiled for 3-5 minutes, before loading the gel.Functional Analysis: Western BlottingReferences:
Product Code:
EXA-X1005 Price (100 µl):
£83.00 excluding VAT
