Highmolecularweightcytokeratinsareexpressedinavarietyofnormalandneoplasticepithelialtissues(1).Inprostate,CKHMW[34βE12]hasbeenshowntobeausefulMarkerofbasalcellsofnormalglandsandprostaticintraepithelialneoplasia(PIN),aprecursorlesiontoprostaticadenocarcinoma;whereasinvasiveprostaticadenocarcinomatypicallylacksabasalcelllayer(2-4).
p63,ahomologofthetumorsuppressorp53,hasbeenidentifiedinproliferatingbasalcellsintheepitheliallayersofavarietyoftissues,includingepidermis,cervix,urotheliumandprostate(5).p63wasdetectedinnucleiofthebasalepitheliuminnormalprostateglands;however,itwasnotexpressedinmalignanttumorsoftheprostate(6).
α-MethylacylcoenzymeAracemase(AMACR),alsoknownasP504S,isaperoxisomalandmitochondrialenzymethatplaysaroleinbileacidsynthesisandβ-oxidationofbranchedchainfattyacids(7).AMACRwasinitiallyidentifiedfromaCDNAlibraryasagenethatisoverexpressedinhumanprostatecancer;withlittleornoexpressioninnormalprostate(8,9).Inimmunohistochemistry,AMACRhasbeenshowntobeaspecificmarkerofprostaticadenocarcinoma(8-11).Additionally,prostateglandsinvolvedinPINhavebeenfoundtoexpressAMACR,whereasAMACRwasnearlyundetectableinbenignglands(11,12).
StudieshaveshownthatcombinationsofCKHMW[34βE12],p63,and/orAMACRmaybeusefulintheevaluationofnormalprostateglands,PINandprostaticadenocarcinoma(13,14).
U.S.Patent8,603,765andpatentspending.
FormerlyknownasPIN-4®
| IntendedUse | IVD |
|---|---|
| Source | MouseMonoclonal,RabbitMonoclonal |
| Clone | 13H4,34BE12,4A4 |
| Isotype | IgG,IgG1/kappa,IgG2a/kappa |
| Antigen | AMACR,CKHMW,p63 |
| Localization | Cytoplasmic,Nuclear |
| PositiveControl | Normalprostateadenocarcinoma |
| SpeciesReactivity | Human;othersnottested |
References:
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