Theconjugatedgoatanti-mousepolymerhorserADIshperoxidase(HRP)andtheconjugatedgoatanti-rabbitpolymeralkalinephosphatase(AP)secondaryantibodiesreactwithbothheavyandlightchainsonmouseandrabbitIgGrespectively.ThisinnovativeHRP-andAP-polymerizationtechnologyprovidesasignificantincreaseinstainingsensitivitywhencomparedtootherconventionalHRP-orAP-conjugatedsecondaryantibodies.Avidin-biotinblockingproceduresarenotnecessarywhenusingtheMACH2conjugatedsecondaryantibodies.Itisspeciallydesignedforarapiddoublestainprocedure.Anantibodycocktailwithamousemonoclonalandarabbitpolyclonal/monoclonalmustbeused.Theoverallstainingprocedurecanbedonewith4majorstepsandinlessthantwohours.Itcanbeusedmanuallyorusedwithautomatedstainers.