Biocare’sPromARKTMseriesintroducesa1-stephorserADIshperoxidase(HRP)polymerdetectionsystemforIHCofratprimaryantibodiesonmousetissues,withminimalcross-reactivity.RatHRP-Polymer,1-Step(Mouseadsorbed)offerstheconvenienceandsensitivityofaone-steppolymerdetectionsystemthatisbiotinandavidinfree.Itisspecificallyformulatedtoimprovespecificityandreducebackgroundstainingonmousetissues.TheeliminationofmouseIgGcanbeapersistentprobleminachievingoptimalstainingofmousetissues.Inadditiontothe1-stepRatHRP-Polymer(Mouseadsorbed)detection,Biocareoffersreagentsthatmayfurtherreduceunwantedbackgroundstaining.AddingXMFactordirectlytoRatHRP-PolymermayfurtherreducebackgroundstainingduetoendogenousmouseIgG.RodentBlockMmayalsobeusedtoreducenonspecificbackgroundstaining(seeindividualdatasheets).BiocarehasalsodevelopedRodentDecloaker,anantigenretrievalsolution,whichisspecificallyformulatedtoreduceand/oreliminatenon-specificbackgroundstainingduetoendogenousmouseandratIgG.TemperaturedependentantigenretrievalprotocolscanbeperformedusingBiocare’sDecloakingChamber(pressurecooker).