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商品描述
Description:Human Noggin, also known as symphalangism 1 (SYM1) or synostoses (multiple) syndrome 1 (SYNS1), GenBank Accession No. NM_005450, amino-acids 28-232(end), disulfide-linked homodimer. MW=~28kDa (monomer).
Source: human 293 cells.
Endotoxin Level: <1 EU/μg.
Biological Activity: measured by Noggin’s ability to inhibit BMP-4-induced alkaline phosphatase production by C2C12 mouse myoblast cells. The ED50 is < 20 ng/ml in the presence of 30 ng/ml of human BMP-4.
Formulation: Lyophilized from a 0.2 μM filtered, aqueous solution.
Reconstitution: Supplied as a lyophilized solid. Reconstitute in sterile water with 0.1% BSA to a final concentration of 0.1 mg/ml. Final formulation will be 50 mM Tris, pH 8.0, 1.2 M NaCl, 2.7 mM KCl, 0.1% BSA.
Stability:≥12 months at –80°C (as a solid). Store reconstituted protein in aliquots at –20°C to –80°C for up to 6 months. Avoid freeze/thaw cycles.
References:
1.Tao, Y.X., et al. (2010). Indian J. Exp. Biol. 48(5):444-52.
2.Mfopou, J.K., et al. (2010). Gastroenter-ology. 138(7):2233- 2245.
3.Chaturvedi, G., et al. (2009). Cell Prolif. 42(4):425-33
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