AKT3, Activehuman, recombinant, N-terminal HIS tag

Size: 10 µg

Description:Recombinant human AKT3 (GenBankAccession No. NM_005465) (a-a 2-end),with N-terminal HIS tag, MW= 60 kDa,expressed in baculovirus expression system.

Specific Activity: 10 pmol/min/ug. Theenzyme reaction is conducted in a buffercontaining 50 mM HEPES (pH7.5), 10 mMMgCl2, 1 mM EGTA, 200 μM ATP, 0.01%Brij-35, and 2 μM substrate (SER/THR 6,Invitrogen) at room temperature for 1 hour.

Application:Useful for the study of enzyme kinetics,screening inhibitors, and selectivityprofiling.

Formulated in: 25 mM Tris-HCl, pH 8.0,138 mM NaCl, 0.05% Tween-20 and 10%glycerol.

Stability: >6 months at –80°C

References:1. Coffer, PJ. et al. Biochem J. 1998 Oct 1; 335 (Pt 1):1-13.2. Anderson, KE. et al. Curr Biol. 1998 Jun4;8(12): 684-91