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Biomol/Rat brain, post natal, Whole Cell Lysate/200 µg/W12-001-MQ6

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货号:W12-001-MQ6
浏览量:55
品牌:Biomol
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商品描述
Product information "Rat brain, post natal, Whole Cell Lysate"
Multi-purpose Whole Cell Lysates produced by Rockland Immunochemicals are derived from tissues and cell lines using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels. Tissues were washed exhaustively with PBS to remove blood and other debris. A lysate was prepared by homogenizing the tissue and washing the cells in cold PBS. Washed cells were incubated at 4° C in modified RIPA buffer containing 150 mM sodium chloride, 50 mM Tris Cl, pH 7.4, 1 mM EDTA, 1.0% NP-40, and 0.5% sodium deoxycholic acid the cells. Protein integrity is ensured using a cocktail of protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases (0.1 mM AEBSF HCl, 0.08 µM Aprotinin, 5 µM Bestatin, 1.5 µM E-64, 2 µM Leupeptin Hemisulfate and 1 µM Pepstatin A). The following phosphatase inhibitors were also added: 1 mM NaF and 1 mM Na3VO4. Cell debris was removed by centrifugation and membrane filtration. Protein concentration was determined by Lowry assay using a commercially available kit. The protein concentration was adjusted to 1 mg/ml using RIPA buffer. Multi-purpose Whole Cell Lysates produced by Rockland Immunochemicals are derived from tissues and cell lines using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels.
Supplier: Rockland Immunochemicals
Supplier-Nr: W12-001-MQ6

Properties

Application: ELISA, IP, WB
Host: Rat
Reactivity: Rat
Format: Liquid

Database Information

Handling & Safety

Storage: -80°C
Shipping: -20°C (International: -20°C)
Caution Our products are for laboratory research use only: Not for administration to humans!
美国生物学(USBiologic)位于美国马萨诸塞州的Swampscott。它的生产能力包括抗生素,抗体,琼脂糖,白蛋白,氨基酸,生化试剂,生物制剂,细胞因子,酶,培养基,分子生物学试剂盒,肽和血清。所有产品均提供经过认证的测试结果。USBio提供了多种抗体。