at 20 bpto 500 bp in 20 bp increments. The bands are all blunt ended, avoiding any artifacts caused by band reannealing. Both polyacrylamide gel electrophoresis and a 3% Metaphor gel resolve bands from 20 bp to 500 bp. All bands on polyacrylamide are very sharp. The lower bands on the Metaphor gel will appear slightly diffuse due to the poorer resolving capability of Metaphor for very small fragments. Polyacrylamide provides 250 loadings per tube, Metaphor provides 125 loadings per tube.
at 100 bp for easy band identification
for accurately sizing smaller PCR products and small sized restriction digests
as a 500 ul stock solution in TE buffer, which is sufficient for 250 loadings for polyacrylamide gels or 125 loadings for Metaphor gels. An additional tube, labeled as "Working Solution", is supplied for making convenient instant use working solutions. The enclosed product information sheet instructs how to mix the working solution.
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