Product Highlights

• Sensitive - reproducible detection of low-copy number templates
• Reproducible – consistent results between technical replicates for increased confidence in results
• Specific - proprietary hot-start modification minimizes non-specific amplification for improved assay reliability in high-throughput assays
• Robust – reliable, accurate detection of DNA and cDNA targets from a broad range of sample types

Product Description

The SensiMix™ SYBR^® Hi-ROX Kit has been developed for highly accurate real-time PCR and has been validated on real-time PCR platforms that require high concentrations of the passive reference dye ROX for normalization of well-to-well differences.

A proprietary, highly-optimized, chemical hot-start PCR enzyme promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range, ensuring that SensiMix SYBR^® Hi-ROX Kit provide the sensitivity and specificity required for demanding assays under standard thermal cycling conditions.

The SensiMix SYBR^® Hi-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

Applications

• Gene expression analysis
• DNA / cDNA target detection
• miRNA profiling / quantification
• Copy number variation (CNV) analysis