BioAssay Systems/EnzyChrom™ Glucose Assay Kit III/100 tests/EGL3-100

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¥6180.00
货号:EGL3-100
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商品描述

EnzyChrom™ Glucose Assay Kit III

EnzyChrom™ Glucose Assay Kit III Catalog No: EGL3-100
Price: $309 Qty:
For orders of 10 or more kits, please call +1-510-782-9988x1 oremail us for best pricing and/or bulk order. Shipping: On Ice Shipment: Fedex ServiceDelivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C
Glucose Assay Kit III (EGL3-100)
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ProtocolSDS

Application

  • For quantitative determination of glucose and evaluation of drug effects on glucose metabolism.

Key Features

  • Sensitive and accurate. Use as little as 20 μL samples. Linear detection range in 96-well plate: 0.03 to 2 mM (0.54 mg/dL to 36 mg/dL) glucose.
  • Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature.

Method

  • OD565nm

Samples

  • Serum, plasma, urine, saliva, milk, culture medium, food, beverages etc

Species

  • All

Size

  • 100 tests

Detection Limit

  • 0.03 mM

Shelf Life

  • 6 months

More Details

  • Glucose(C6H12O6) is a key diagnostic parameter for many metabolic disorders. Increased glucose levels have been associated with diabetes mellitus, hyperactivity of thyroid, pituitary and adrenal glands. Decreased levels are found in insulin secreting tumors, myxedema, hypopituitarism and hypoadrenalism. Simple, direct and high-throughput assays for measuring glucose concentrations find wide applications in research and drug discovery. BioAssay Systems glucose assay kit III uses a single Working Reagent that combines the enzyme reaction and color reaction in one step. The color intensity of the reaction product at 565 nm is directly proportional to the glucose concentration in the sample.

What are the differences between the three EnzyChrom Glucose assay kits?

EBGL - best at detecting low levels of glucose (as low as 5 µM)EGL2 - best at measuring high levels of glucose using a low wavelength filter (340 nm)EGL3 - best at measuring high levels of glucose using a high wavelength filter (565 nm)

Which of the three EnzyChrom Glucose assay kits can be used with urine, saliva, or serum samples?

EBGL - Not recommended for urine, saliva, or serum samplesEGL2 - Compatible with urine and serum samples, not recommended for saliva samplesEGL3 - Recommended for urine, saliva, and serum samples*Sample blanks are to be used when testing urine samples with EGL2 or EGL3.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
BioAssay Systems的EnzyFluo™ERK磷酸化检测试剂盒的有丝分裂原活化蛋白激酶(MAPK / ERK)途径在细胞增殖,分化和迁移中起关键作用。有丝分裂原刺激最终导致ERK1(T202 / Y204)和ERK2(T185 / Y187)磷酸化。MAPK / ERK级联为癌症治疗的发展提出了许多有趣的药物靶标。BioAssay Systems基于细胞的ELISA可测量全细胞中双重磷酸化的ERK1 / 2,并将信号归一化至总蛋白质含量。这种简单有效的测定方法消除了细胞裂解液制备的需要,可用于研究激酶信号传导和激酶抑制剂对细胞的作用。在该测定中,细胞在96孔板中生长,并用配体或药物处理。然后将细胞固定并在孔中透化。使用荧光ELISA进行ERK1 / 2磷酸化(pERK),然后在每个孔中测量总蛋白。