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蚂蚁淘在线 / 品牌中心 / BioAssay Systems / BioAssay Systems/QuantiChrom™ Alcohol Dehydrogenase Assay Kit/100 tests/DADH-100
商品描述
QuantiChrom™ Alcohol Dehydrogenase Assay Kit
- Product Overview
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- Assay Service
ProtocolSDS
Application
- For quantitative determination of alcohol dehydrogenase activity and evaluation of drug effects on its metabolism.
Key Features
- Fast and sensitive. Linear detection range (20 μL sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 min reaction.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method
- OD565nm
Samples
- Biological (e.g. plasma, serum, urine, tissue and culture media)
Species
- All
Size
- 100 tests
Detection Limit
- 0.4 U/L
Shelf Life
- 6 months
More Details
- ALCOHOL DEHYDROGENASE (ADH) is an oxidoreductase which catalyzes the interconversion of alcohols and aldehydes or ketones. ADH is important in humans and other organisms for the break down of alcohols which may otherwise be toxic. In yeast and some bacteria, ADHs catalyze the opposite reaction and produce alcohol as part of fermentation. BioAssay Systems non-radioactive, colorimetric ADH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.