| Cytochalasin Jalters mitotic spindle microtubule organization and kinetochore structure |
Sample solution is provided at 25 µL, 10mM.
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Chemical structure
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| Cas No. | 53760-20-6 | SDF | Download SDF |
| Synonyms | N/A | ||
| Chemical Name | 2,3,3a,4,5,6,6a,9,10,11,12,15-dodecahydro-6,12,15-trihydroxy-4,10,12-trimethyl-5-methylene-3-(phenylmethyl)-1H-cycloundec[d]isoindol-1-one | ||
| Canonical SMILES | O=C1N[C@@H](CC2=CC=CC=C2)[C@]([C@]31[C@H](O)/C=C/[C@](C)(O)C[C@@H](C)C/C=C/[C@@]3([H])[C@@H]4O)([H])[C@H](C)C4=C | ||
| Formula | C28H37NO4 | M.Wt | 451.6 |
| Solubility | Soluble in ethanol;Soluble in methanol;Soluble in DMSO;Soluble in dimethyl formamide | Storage | Store at -20°C |
| Physical Appearance | A white lyophilisate | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
Cytochalasin J can alter mitotic spindle microtubule organization and kinetochore structure.
The cytochalasins are cell-permeable fungal metabolites inhibiting actin polymerization, which can alter diverse processes including cell movement, growth, phagocytosis, degranulation, and secretion.
In vitro: Cytochalasin J was identified as a deacetylated analog of cytochalasin H that weakly inhibited actin assembly but significantly altered mitotic spindle microtubule organization and kinetochore structure [1]. In a previous study, cytochalasin J treatment of prometaphase cells reduced the number of kMTs and the size and organization of the kinetochore lamina. Moreover, in approximately 30% of cells treated with cytochalasin J, the failure of a small number of chromosomes to attach to spindle fibers could be documented. These chromosomes showed a significant change in the organization of the kinetochore laminae. Light microscopic analyses of cells treated with cytochalasin J revealed loss of chromosome congression, with chromsomes usually located at the periphery of the spindle. Cells treated with 10 microg/ml cytochalasin J for 10 min and released into tissue culture medium showed a resumption of chromosome motion within a few minutes, both during congression and anaphase [2].
In vivo: Up to now, there is no animal in vivo data reported.
Clinical trial: So far, no clinical study has been conducted.
References:[1] Walling, E. A.,Krafft, G.A. and Ware, B.R. Actin assembly activity of cytochalasins and cytochalasin analogs assayed using fluorescence photobleaching recovery. Archives of Biochemistry and Biophysics 264(1), 321-332 (1988).[2] Wrench, G. A. and Snyder, J.A. Cytochalasin J treatment significantly alters mitotic spindle microtubule organization and kinetochore structure in PtK1 cells. Cell Motility and the Cytoskeleton 36(2), 112-124 (1997).
