| Blasticidin S HClantibiotic, inhibits protein synthesis |
Sample solution is provided at 25 µL, 10mM.
Nature.2017 Jan 19;541(7637):417-420.
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Chemical structure
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| Cas No. | 3513-03-9 | SDF | Download SDF |
| Synonyms | N/A | ||
| Chemical Name | (2S,3S,6R)-3-[[(3R)-3-amino-5-[carbamimidoyl(methyl)amino]pentanoyl]amino]-6-(4-amino-2-oxopyrimidin-1-yl)-3,6-dihydro-2H-pyran-2-carboxylic acid;hydrochloride | ||
| Canonical SMILES | CN(CCC(CC(=O)NC1C=CC(OC1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl | ||
| Formula | C17H27ClN8O5 | M.Wt | 458.9 |
| Solubility | Limited solubility, soluble in H2O | Storage | Store at -20°C |
| Physical Appearance | White solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
Blasticidin S HCl is an antibiotic inhibiting protein synthesis [1].
Blasticidin S is isolated from Streptomyces griseochromogenes and inhibits protein synthesis in both prokaryotic and eukaryotic cells.
Blasticidin S HCl is an antibiotic inhibiting protein synthesis. In mouse leukemia L5178Y cells, blasticidin S inhibited protein synthesis and caused the degradation of presumptive mRNA [1]. Also, blasticidin S inhibited ribonuclease P activity from Dictyostelium discoideum and inhibited tRNA maturation in a dose dependent way [2]. In S. cerevisiae, blasticidin S inhibited protein synthesis induced by galactose. In Aspergillus parasiticus, blasticidin S inhibited aflatoxin production without inhibition of fungal growth. In Aspergillus flavus, blasticidin S significantly reduced aflatoxin biosynthetic enzymes [3]. Blasticidin S efficiently bound Cu2+ ions and protected DNA from metal-induced damage [4]. Blasticidin S enhanced the binding of tRNA to the P site of the large ribosomal subunit and slowed spontaneous intersubunit rotation. Blasticidin S inhibited peptide bond formation and peptidyl-tRNA hydrolysis [5].
References:[1]. Takenaka K, Endo H, Kuwano M. The synthesis and degradation of presumptive messenger RNA in cultured mouse leukemia cells during the inhibition of protein synthesis. Biochim Biophys Acta, 1978, 521(1): 295-307.[2]. Stathopoulos C, Tsagla A, Tekos A, et al. Effect of peptidyltransferase inhibitors on ribonuclease P activity from Dictyostelium discoideum. Effect of antibiotics on RNase P. Mol Biol Rep, 2000, 27(2): 107-111.[3]. Yoshinari T, Noda Y, Yoda K, et al. Inhibitory activity of blasticidin A, a strong aflatoxin production inhibitor, on protein synthesis of yeast: selective inhibition of aflatoxin production by protein synthesis inhibitors. J Antibiot (Tokyo), 2010, 63(6): 309-314. [4]. Stokowa-Sołtys K, Jeżowska-Bojczuk M. A rice fungicide blasticidin S efficiently binds Cu(II) ions and prevents DNA from metal-induced damage. J Inorg Biochem, 2013, 127: 73-78.[5]. Svidritskiy E, Ling C, Ermolenko DN, et al. Blasticidin S inhibits translation by trapping deformed tRNA on the ribosome. Proc Natl Acad Sci U S A, 2013, 110(30): 12283-12288.
