Cell lines

primary neuronal cultures; the neuroblastoma SH-SY5Y cell line

Preparation method

The solubility of this compound in DMSO is >9.7mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

0.1, 1, and 10 μM; 50 μM

Applications

In primary neuronal cultures, resveratrol (0.1, 1, and 10 μM) inhibited OGD/reperfusion-induced apoptosis and inhibited the overexpression of caspase-3 and caspase-12 mRNA in a concentration-dependent way. In the neuroblastoma SH-SY5Y cell line, resveratrol (50 μM) inhibited excess dopamine-induced cell death by ameliorating intracellular oxidative stress and increasing the activity of prosurvival gene Bcl-2.

Animal models

rats subjected to myocardial ischemia

Dosage form

2.5, 5.0, 25 or 50 mg/kg; fed for 14 days by gavaging

Application

In rats subjected to myocardial ischemia, resveratrol (2.5, 5.0 mg/kg) exhibited cardioprotection by improved post-ischemic ventricular recovery and reduction of myocardial infarct size and cardiomyocyte apoptosis. However, 25 or 50 mg/kg dose of resveratrol inhibited cardiac function and increased myocardial infarct size and number of apoptotic cells.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Nilendra Singh, Megha Agrawal, and Sylvain Doré. Neuroprotective Properties and Mechanisms of Resveratrol in in Vitro and in Vivo Experimental Cerebral Stroke Models. ACS Chem Neurosci. 2013 Aug 21; 4(8): 1151–1162.

[2]. Dudley J1, Das S, Mukherjee S, et al. Resveratrol, a unique phytoalexin present in red wine, delivers either survival signal or death signal to the ischemic myocardium depending on dose. J Nutr Biochem. 2009 Jun;20(6):443-52.