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ApexBio/Caspofungin Acetate/10mM (in 1mL DMSO)/B2083

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¥6800.00
货号:B2083
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品牌:ApexBio
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Caspofungin Acetatelipopeptide antifungal drug

Catalog No.B2083
SizePriceStockQty
10mM (in 1mL DMSO)
$330.00
In stock
5mg
$45.00
In stock
10mg
$80.00
In stock
50mg
$340.00
In stock

Tel: +1-832-696-8203

Email: sales@apexbt.com

Worldwide Distributors

Sample solution is provided at 25 µL, 10mM.

Product Citations

1. Granger BL. "Accessibility and contribution to glucan masking of natural and genetically tagged versions of yeast wall protein 1 of Candida albicans." PLoSOne. 2018 Jan 12;13(1):e0191194.PMID:29329339

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Quality Control

Quality Control & MSDS

View current batch:
    Purity = 98.06%
  • COA (Certificate Of Analysis)
  • HPLC
  • NMR (Nuclear Magnetic Resonance)
  • MSDS (Material Safety Data Sheet)
  • Datasheet

Chemical structure

Caspofungin Acetate

Related Biological Data

Caspofungin Acetate

Protocol

Cell experiment [1]:

Cell lines

Aspergillus fumigates

Preparation method

The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

32 μg/ml for 6 h; or 2 μg/ml for 12 h

Applications

Caspofungin suppressed the synthesis of cell wall β-1,3-glucan, which triggered a compensatory stimulation of chitin synthesis. Caspofungin induced morphological changes in Aspergillus fumigates. Moreover, Treatment with caspofungin induced ChsG-dependent upregulation of chitin synthesis and the formation of chitin-rich microcolonies in Aspergillus fumigates.

References:

1Walker, L. A., Lee, K. K., Munro, C. A. and Gow, N. A. (2015) Caspofungin Treatment of Aspergillus fumigatus Results in ChsG-Dependent Upregulation of Chitin Synthesis and the Formation of Chitin-Rich Microcolonies. Antimicrob Agents Chemother. 59, 5932-5941

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Chemical Properties

Cas No. 179463-17-3SDF Download SDF
Synonyms N/A
Chemical Name acetic acid compound with (Z)-N-((2R,6S,7Z,9S,11R,12S,13E,14aS,15S,20S,21Z,23S,24Z,25aS)-20-((R)-3-amino-1-hydroxypropyl)-12-((2-aminoethyl)amino)-23-((1S,2S)-1,2-dihydroxy-2-(4-hydroxyphenyl)ethyl)-2,8,11,14,15,22,25-heptahydroxy-6-((R)-1-hydroxyethyl)-5
Canonical SMILES CCC(C)CC(C)CCCCCCCCC(=O)NC1CC(C(NC(=O)C2C(CCN2C(=O)C(NC(=O)C(NC(=O)C3CC(CN3C(=O)C(NC1=O)C(C)O)O)C(C(C4=CC=C(C=C4)O)O)O)C(CCN)O)O)NCCN)O.CC(=O)O.CC(=O)O
Formula C56H96N10O19 M.Wt 1213.42
Solubility ≥60.671mg/mL in DMSO Storage Store at -20°C
Physical AppearanceA solidShipping ConditionEvaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Background

Caspofungin acetate(MK-0991), a new antifungal agent with minimal toxicity, inhibits synthesis of 1,3-β-D-glucan, an essential component of the fungal cell wall [1].

In vitro: Incubation ofA. fumigatuwith a single dose of caspofungin affected the same proportion of apical and subapical branching cells for up to 72 h.The cells at the active centers for new cell wall synthesis withinA. fumigateshyphae are killed when they are exposed to caspofungin [1]. Caspofungin acetate irreversibly inhibited the enzyme 1,3-β-D-glucan synthase, preventing the formation of glucan polymers and disrupting the integrity of the fungal cell wall. The elimination half-life of caspofungin acetate was 9-10 hours [2]. Caspofungin showed activity againstAspergillusspp. as well as a variety ofCandidaspp[3]. Growth kinetic studies of caspofungin acetate against Candida albicans and Candida tropicalis isolates showed that caspofungin acetate exhibited fungicidal activity (i.e., a 99% reduction in viability) within 3 to 7 h at concentrations ranging from 0.06 to 1 μg/ml (0.25 to 4 times the MIC)[4].The minimal inhibitory concentration for 90% inhibition of Candida species by caspofungin acetate were as follows:C. albicans 0.5 μg/mL (range, 0.25-0.5), C. glabrata 1.0 μg/mL (range, 0.25-2.0), C. tropicalis 1.0 μg/mL (range, 0.25-1.0), C. parapsilosis 0.5 μg/mL (range, 0.25-1.0), and C. krusei 2.0 μg/mL (range, 0.5-2.0)[4].

Clinical trials: In a trial of 128 patients with Candida esophagitis, clinical response rates were higher with caspofungin acetate 50 or 70 mg/day (85%) than with amphotericin B 0.5 mg/kg/day (67%)[2].

References:[1]. Bowman J C, Hicks P S, Kurtz M B, et al. The antifungal echinocandincaspofungin acetate kills growing cells of Aspergillus fumigatus in vitro[J]. Antimicrobial agents and chemotherapy, 2002, 46(9): 3001-3012.[2]. Hoang A. Caspofungin acetate: an antifungal agent[J]. American journal of health-system pharmacy, 2001, 58(13): 1206-1214.[3]. Pacetti S A, Gelone S P. Caspofungin acetate for treatment of invasive fungal infections[J]. Annals of Pharmacotherapy, 2003, 37(1): 90-98.[4]. Bartizal K, Gill C J, Abruzzo G K, et al. In vitro preclinical evaluation studies with the echinocandin antifungal MK-0991 (L-743,872)[J]. Antimicrobial Agents and Chemotherapy, 1997, 41(11): 2326-2332.

ApexBio的3X FLAG Peptide FLAG标签系统利用与目标蛋白质1融合的短而亲水的8个氨基酸的肽段。FLAG肽与抗体M1结合。结合是钙依赖性方式2还是非依赖性3仍存在争议。该系统的缺点是单克隆抗体纯化基质不如其他基质稳定。通常,可以用特异性单克隆抗体检测小标签。为了改善对FLAG标签的检测,已经开发了3x FLAG系统。这种三级FLAG表位是亲水的,长22个氨基酸,可以检测到高达10 fmol的表达融合蛋白。激烈热球菌的带有FLAG标签的麦芽糖糊精结合蛋白已被结晶4,其晶体质量与未标记蛋白的晶体质量非常相似。 最后,可以通过用肠激酶处理去除FLAG标签,肠激酶对肽序列5的5个C末端氨基酸具有特异性。