| ML-336inhibit a VEEV-induced cytopathic effect |
Sample solution is provided at 25 µL, 10mM.
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- Purity = 98.00%
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Chemical structure
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| Cas No. | 1613465-33-0 | SDF | Download SDF |
| Chemical Name | 2-[(E)-(1,4-dimethyl-2-piperazinylidene)amino]-5-nitro-N-phenyl-benzamide | ||
| Canonical SMILES | CN1CCN(C)C/C1=NC2=C(C(NC3=CC=CC=C3)=O)C=C([N+]([O-])=O)C=C2 | ||
| Formula | C19H21N5O3 | M.Wt | 367.4 |
| Solubility | ≤0.2mg/ml in ethanol;10mg/ml in DMSO;20mg/ml in dimethyl formamide | Storage | Store at -20°C |
| Physical Appearance | A crystalline solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
IC50: 32, 20, and 41 nM for TC-83, V3526, and Trinidad donkey strains, respectively.
ML-336 is an inhibitor for VEEV-induced cytopathic effect.
Alphaviruses like Venezuelan Equine Encephalitis Virus (VEEV) are positive-sense, enveloped, single stranded RNA viruses that are widely geographically distributed. VEEVs are found to be arthropod-borne viruses that are known to cause arthritis, encephalitis, rash, as well as death in humans.
In vitro: ML336 was identified as a first-in-class probe that inhibited a VEEV-induced cytopathic effect in three strains of the virus, which were V3526, TC-83, and Trinidad donkey, in the low nanomolar range without having cytotoxicity (> 50 μM, selectivity index > 1500). In addition, ML336 could reduce viral titer (more than 7.2 log) dramatically below a 1 μM drug concentration. Furthermore, ML336 showed a favorable in vitro PK profile that included moderate blood-brain barrier permeability. More importantly, ML336 seemed to be able to target the VEEV [1].
In vivo: Currently, there is no animal in vivo data reported.
Clinical trial: So far, no clinical study has been conducted.
Reference:[1] Chung D et al. ML336: Development of Quinazolinone-Based Inhibitors Against Venezuelan Equine Encephalitis Virus (VEEV). Probe Reports from the NIH Molecular Libraries Program. Bethesda (MD): National Center for Biotechnology Information (US); 2010-2012 Dec 17.
