Benefits

• Eliminates errors and reduces experimentalprocessing time
• Auto data logging eliminates the need totrack data by hand
• The small footprint allows more benchspace
• Easy calibration and verification
• Footswitch for hands-free recording
• Prevent data loss with auto save and datarecovery when battery is low
• TEER is easily computed by applying a unitarea formula to the resistance

Applications

• Measure epithelial or endothelial tissues forconfluence, TEER and potential difference
• Permeability, conductance and drug studies
• Continuous digital monitoring of a target membrane
• Common studies
• Blood-brain barrier transport
• Lung epithelial tissue studies
• Intestinal tissue studies
• Skin studies

EVOM3 For TeerMeasurement

The EVOM3 delivers improved workflow efficiency, more stable and repeatablemeasurements versus traditional Trans Epithelial Electrical Resistance (TEER) meters.Providing users with vital feedback during experiment measurements, the EVOM3’s large screenoffers a range of informational views. The new graphical displays for trend analysis and measurement values helpsscientists deliver simple, stepwise methodology during experimental measurements. The touch screen interface providesusers an intuitive, easy-to-use menu for configuration.Eliminating the need to log data by hand, the EVOM3 writes the resistance or voltage information to a USB drive in CSVformat for easy transfer to spreadsheets and data analysis programs. When used with the footswitch it enables handsfreerecording of measurements.At the heart of the EVOM3 is our latest processor and circuitry, providing users with quick, easy and reliable readingsdue to its fast stabilization, automatic twenty times sampling average and low noise design. The auto ranging resistancefeature allows for fast resistance measurements, and an over-range display feature eliminates false readings. The EVOM3has adjustable current levels in three fixed ranges with two lower ranges for sensitive membranes and high resistanceranges up to 100 KΩ.

TEER Measurement Electrode

The STX2-PLUS electrode was designed for easy insertion into many 24-well plates. Itis location re-placeable in the insert for repeatable and consistent measurements. Thenew shielded electrodes are now designed to minimize electrical interference and to bemore easily maintained.

• STX2-PLUS new electrode designed for 12 and 24-wellplates.
• Weighted self-standing electrode for hands-free stablemeasurements
• Shielded cable to minimize electrical and cellphone interference

System Components

NOTE: A 99672 EVOM2 to EVOM3 Electrode Adapter is soldseparately. The STX2, STX3 and all STX100s require the use ofthis adapter with the EVOM3.

STX2-Plus

STX2-PlusBenefits

• Keyed electrode base for repeatable placementgives more consistent results, eliminating theneed for multiple readings.
• Easy to maintain

How Does the EVOM3 Work?

Confluence of a cellular monolayer is determined by an increase or a plateau in tissue resistance detected using theunique electronic circuit of the EVOM3 and the new STX2-PLUS electrode. The EVOM3 qualitatively measures cellmonolayer health and quantitatively measures cell confluence. The EVOM3 produces a low AC current that avoidselectrode metal deposits and adverse effects on tissues which can otherwise be caused by higher DC currents. TheEVOM3 uses low current and voltages and is designed for non-destructive testing for epithelial monolayer confluencein cell cultures. In addition, resistance readings are unaffected by membrane capacitance or membrane voltage. Theaccuracy and repeatability of the EVOM3-STX2-PLUS system makes this instrument ideal for permeability, PD and otherdetailed membrane studies.

Electrodes For TEER (Epithelial) Measurement

*(Requires 99672 for use with the EVOM3)

ENDOHM Chambers For Endothelial/EpithelialMeasurement

NEW EndOhm chambers include the EVOM3 cable 99916.

TEER measurement techniques for in vitro barrier model systems