MICRO2T SMARTouch Instruction Manual

Nanoliter2010 Instruction Manual

Nanoliter2010 Quick Start Guide

NL2010MC2T Quick Start Guide 

FAQ for Nanoliter 2010 Injector Leaks

FAQ:

My Nanoliter2010 has liquid leaking out or I can see bubbles forming inside the pipette. What can cause this and what action can I take to correct the problem?

Answer:

1) Easy to fix, as it is probably the seal rings. Check to make sure the seal rings are assembled in the correct sequence.

2) The plunger seal has a conical shaped hole in the center. The seal should be  installed with the larger end of the hole pushed onto the plunger first. This means the smaller end of the hole is facing toward the tip of the plunger. This is shown graphically in the lower diagram below. Pay special attention to the notes in red.  If the plunger seal slides off easily by gravity when the Nanoliter Injector is held vertically pointing downward, the seal hole is too large and it will leak. Replace the seal.

The plunger seal should not slide off the plunger shaft under gravity when  tested by holding the nanoliter injector vertically with the tip pointing downward (with no other seals installed). There should be some friction when pushing the plunger seal onto the plunger and it should grip the plunger slightly. It should not be loose.

3) The orientation of the plastic spacer and large glass seal (reddish color with large hole for capillary glass) must be oriented correctly as shown. The shank end of the capillary must be correctly seated into the recess in the plastic spacer.

4) The seals should and capillary glass should be clean. If the seals are oily, they are more likely to leak. Use isopropyl alcohol to clean the seals, and if necessary immerse them in it to get the oil out of the hole.

5) Tighten the cap securely.

503043 Universal Power Supply (12V DC), 5.5x2.1mm

Amir, D., Yakir, E., & Bohbot, J. D. (2019). Indolergic receptors of the elephant mosquito Toxorhynchites amboinensis. BioRxiv, 513044. https://doi.org/10.1101/513044

Li, R., Huang, Y., Zhang, Q., Zhou, H., Jin, P., & Ma, F. (2019). The miR-317 functions as a negative regulator of Toll immune response and influences Drosophila survival. Developmental & Comparative Immunology, 95, 19–27. https://doi.org/10.1016/J.DCI.2019.01.012 

Li, R., Weng, J., Wang, X., Meng, Q., Wang, Y., & Sun, J. (2019). Bursicon homodimers induce innate immune by activating the expression of anti-microbial peptide genes in the shrimp Neocaridina heteropoda. Fish & Shellfish Immunology, 84, 906–911. https://doi.org/10.1016/J.FSI.2018.10.080

Li, X., Liu, F.-Z., Cai, W.-L., Zhao, J., Hua, H.-X., & Zou, Y.-L. (2019). The function of spineless in antenna and wing development of the brown planthopper, Nilaparvata lugens. Insect Molecular Biology, 28(2), 196–207. https://doi.org/10.1111/imb.12538

Li, Y., Liu, Z., Guo, Q., & Luo, M. (2019). Long-term Fiber Photometry for Neuroscience Studies. Neuroscience Bulletin, 35(3), 425–433. https://doi.org/10.1007/s12264-019-00379-4

Minakata, T., Inagaki, A., Yamamura, A., Yamamura, H., Sekiya, S., & Murakami, S. (2019). Calcium-Sensing Receptor Is Functionally Expressed in the Cochlear Perilymphatic Compartment and Essential for Hearing. Frontiers in Molecular Neuroscience, 12, 175. https://doi.org/10.3389/fnmol.2019.00175

Scala, R., Maqoud, F., Angelelli, M., Latorre, R., Perrone, M., Scilimati, A., & Tricarico, D. (2019). Zoledronic Acid Modulation of TRPV1 Channel Currents in Osteoblast Cell Line and Native Rat and Mouse Bone Marrow-Derived Osteoblasts: Cell Proliferation and Mineralization Effect. Cancers, 11(2), 206. https://doi.org/10.3390/cancers11020206

Cambridge, S. (n.d.). Photoswitching proteins : methods and protocols.

Sato, T. R., & Svoboda, K. (n.d.). The Functional Properties of Barrel Cortex Neurons Projecting to the Primary Motor Cortex. https://doi.org/10.1523/JNEUROSCI.3774-09.2010

Tappert, L., Pokorny, T., Hofferberth, J., & Ruther, J. (2017). Sublethal doses of imidacloprid disrupt sexual communication and host finding in a parasitoid wasp. Scientific Reports, 7(1), 42756. https://doi.org/10.1038/srep42756

Garland, C. J., Bagher, P., Powell, C., Ye, X., Lemmey, H. A. L., Borysova, L., & Dora, K. A. (2017). Voltage-dependent Ca ²⁺ entry into smooth muscle during contraction promotes endothelium-mediated feedback vasodilation in arterioles. Science Signaling, 10(486), eaal3806. https://doi.org/10.1126/scisignal.aal3806

Suzuki, J., Hashimoto, K., Xiao, R., Vandenberghe, L. H., & Liberman, M. C. (2017). Cochlear gene therapy with ancestral AAV in adult mice: complete transduction of inner hair cells without cochlear dysfunction. Scientific Reports, 7(1), 45524. https://doi.org/10.1038/srep45524

Tan, Q.-Q., Liu, W., Zhu, F., Lei, C.-L., Hahn, D. A., & Wang, X.-P. (2017). Describing the Diapause-Preparatory Proteome of the Beetle Colaphellus bowringi and Identifying Candidates Affecting Lipid Accumulation Using Isobaric Tags for Mass Spectrometry-Based Proteome Quantification (iTRAQ). Frontiers in Physiology, 8, 251. https://doi.org/10.3389/fphys.2017.00251

Shi, Z., Liang, H., & Hou, Y. (2017). Functional analysis of a NF-κB transcription factor in the immune defense of Oriental fruit fly, Bactrocera dorsalis Hendel (Diptera: Tephritidae). Bulletin of Entomological Research, 107(02), 251–260. https://doi.org/10.1017/S0007485316000845

Chen, C., Yang, H., Tang, B., Yang, W.-J., & Jin, D.-C. (2017). Identification and functional analysis of chitinase 7 gene in white-backed planthopper, Sogatella furcifera. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 208–209, 19–28. https://doi.org/10.1016/j.cbpb.2017.03.002

Li, S., Shen, L., Sun, L., Xu, J., Jin, P., Chen, L., & Ma, F. (2017). Small RNA-Seq analysis reveals microRNA-regulation of the Imd pathway during Escherichia coli infection in Drosophila. Developmental & Comparative Immunology, 70, 80–87. https://doi.org/10.1016/j.dci.2017.01.008

Lynagh, T., Flood, E., Boiteux, C., Wulf, M., Komnatnyy, V. V, Colding, J. M., … Pless, S. A. (2017). A selectivity filter at the intracellular end of the acid-sensing ion channel pore. ELife, 6. https://doi.org/10.7554/eLife.24630

Garriga, M., Raddatz, N., Véry, A.-A., Sentenac, H., Rubio-Meléndez, M. E., González, W., & Dreyer, I. (2017). Cloning and functional characterization of HKT1 and AKT1 genes of Fragaria spp.—Relationship to plant response to salt stress. Journal of Plant Physiology, 210, 9–17. https://doi.org/10.1016/j.jplph.2016.12.007

Smith, I. T., Townsend, L. B., Huh, R., Zhu, H., & Smith, S. L. (2017). Stream-dependent development of higher visual cortical areas. Nature Neuroscience, 20(2), 200–208. https://doi.org/10.1038/nn.4469

Li, S., Li, Y., Shen, L., Jin, P., Chen, L., & Ma, F. (2017). miR-958 inhibits Toll signaling and Drosomycin expression via direct targeting of Toll and Dif in Drosophila melanogaster. American Journal of Physiology-Cell Physiology, 312(2), C103–C110. https://doi.org/10.1152/ajpcell.00251.2016

Moreno-Juan, V., Filipchuk, A., Antón-Bolaños, N., Mezzera, C., Gezelius, H., Andrés, B., … López-Bendito, G. (2017). Prenatal thalamic waves regulate cortical area size prior to sensory processing. Nature Communications, 8, 14172. https://doi.org/10.1038/ncomms14172

Chouchane, M., Melo de Farias, A. R., Moura, D. M. de S., Hilscher, M. M., Schroeder, T., Leão, R. N., & Costa, M. R. (2017). Lineage Reprogramming of Astroglial Cells from Different Origins into Distinct Neuronal Subtypes. Stem Cell Reports, 9(1), 162–176. https://doi.org/10.1016/j.stemcr.2017.05.009

Lynagh, T., Komnatnyy, V. V, & Pless, S. A. (2017). Unique Contributions of an Arginine Side Chain to Ligand Recognition in a Glutamate-gated Chloride Channel. The Journal of Biological Chemistry, 292(9), 3940–3946. https://doi.org/10.1074/jbc.M116.772939

Lesicko, A. M. H., Hristova, T. S., Maigler, K. C., & Llano, D. A. (2016). Connectional Modularity of Top-Down and Bottom-Up Multimodal Inputs to the Lateral Cortex of the Mouse Inferior Colliculus. The Journal of Neuroscience : The Official Journal of the Society for Neuroscience, 36(43), 11037–11050. https://doi.org/10.1523/JNEUROSCI.4134-15.2016

Dekel, A., Pitts, R. J., Yakir, E., & Bohbot, J. D. (2016). Evolutionarily conserved odorant receptor function questions ecological context of octenol role in mosquitoes. Scientific Reports, 6(1), 37330. https://doi.org/10.1038/srep37330

Hulsey, D. R., Hays, S. A., Khodaparast, N., Ruiz, A., Das, P., Rennaker, R. L., & Kilgard, M. P. (2016). Reorganization of Motor Cortex by Vagus Nerve Stimulation Requires Cholinergic Innervation. Brain Stimulation, 9(2), 174–181. https://doi.org/10.1016/j.brs.2015.12.007

Tervo, D. G. R., Hwang, B.-Y., Viswanathan, S., Gaj, T., Lavzin, M., Ritola, K. D., … Karpova, A. Y. (2016). A Designer AAV Variant Permits Efficient Retrograde Access to Projection Neurons. Neuron, 92(2), 372–382. https://doi.org/10.1016/j.neuron.2016.09.021

Kim, J.-H., Jung, A.-H., Jeong, D., Choi, I., Kim, K., Shin, S., … Lee, S.-H. (2016). Selectivity of Neuromodulatory Projections from the Basal Forebrain and Locus Ceruleus to Primary Sensory Cortices. The Journal of Neuroscience, 36(19), 5314–5327. https://doi.org/10.1523/JNEUROSCI.4333-15.2016

Ali, A., Raddatz, N., Aman, R., Kim, S., Park, H. C., Jan, M., … Yun, D.-J. (2016). A Single Amino-Acid Substitution in the Sodium Transporter HKT1 Associated with Plant Salt Tolerance. Plant Physiology, 171(3), 2112–2126. https://doi.org/10.1104/pp.16.00569

Strobl, M.-T. J., Freeman, D., Patel, J., Poulsen, R., Wendler, C. C., Rivkees, S. A., & Coleman, J. E. (2016). Opposing Effects of Maternal Hypo- and Hyperthyroidism on the Stability of Thalamocortical Synapses in the Visual Cortex of Adult Offspring. Cerebral Cortex, 27(5), bhw096. https://doi.org/10.1093/cercor/bhw096

Plautz, C. Z., Williams, H. C., & Grainger, R. M. (2016). Functional Cloning Using a <em>Xenopus</em> Oocyte Expression System. Journal of Visualized Experiments, (107), e53518–e53518. https://doi.org/10.3791/53518

Vinken, M., & Johnstone, S. R. (Eds.). (2016). Gap Junction Protocols (Vol. 1437). New York, NY: Springer New York. https://doi.org/10.1007/978-1-4939-3664-9

Eom, K., Im, C., Hwang, S., Eom, S., Kim, T.-S., Jeong, H. S., … Kim, S. J. (2016). Synergistic combination of near-infrared irradiation and targeted gold nanoheaters for enhanced photothermal neural stimulation. Biomedical Optics Express, 7(4), 1614. https://doi.org/10.1364/BOE.7.001614

Schuette, S. R. M., Fernández-Fernández, D., Lamla, T., Rosenbrock, H., & Hobson, S. (2016). Overexpression of Protein Kinase Mζ in the Hippocampus Enhances Long-Term Potentiation and Long-Term Contextual But Not Cued Fear Memory in Rats. The Journal of Neuroscience : The Official Journal of the Society for Neuroscience, 36(15), 4313–4324. https://doi.org/10.1523/JNEUROSCI.3600-15.2016

Chouchane, M. (2016). Reprogramming of distinct astroglial populations into specific neuronal subtypes in vitro and in vivo. Retrieved from https://repositorio.ufrn.br/jspui/handle/123456789/21277

Florez-Paz, D., Bali, K. K., Kuner, R., & Gomis, A. (2016). A critical role for Piezo2 channels in the mechanotransduction of mouse proprioceptive neurons. Scientific Reports, 6(1), 25923. https://doi.org/10.1038/srep25923

Laprell, L., Repak, E., Franckevicius, V., Hartrampf, F., Terhag, J., Hollmann, M., … Trauner, D. (2015). Optical control of NMDA receptors with a diffusible photoswitch. Nature Communications, 6(1), 8076. https://doi.org/10.1038/ncomms9076

Saha-Shah, A., Weber, A. E., Karty, J. A., Ray, S. J., Hieftje, G. M., & Baker, L. A. (2015). Nanopipettes: probes for local sample analysis. Chem. Sci., 6(6), 3334–3341. https://doi.org/10.1039/C5SC00668F

Nawata, C. M., Dantzler, W. H., & Pannabecker, T. L. (2015). Alternative channels for urea in the inner medulla of the rat kidney. American Journal of Physiology-Renal Physiology, 309(11), F916–F924. https://doi.org/10.1152/ajprenal.00392.2015

Schweikhard, E. S., Kempson, S. A., Ziegler, C., & Burckhardt, B. C. (2014). Mutation of a single threonine in the cytoplasmic NH ₂ terminus disrupts trafficking of renal betaine-GABA transporter 1 during hypertonic stress. American Journal of Physiology-Renal Physiology, 307(1), F107–F115. https://doi.org/10.1152/ajprenal.00085.2014

Mellott, J. G., Foster, N. L., Ohl, A. P., & Schofield, B. R. (2014). Excitatory and inhibitory projections in parallel pathways from the inferior colliculus to the auditory thalamus. Frontiers in Neuroanatomy, 8, 124. https://doi.org/10.3389/fnana.2014.00124

Liu, Y., Keefe, K., Tang, X., Lin, S., & Smith, G. M. (2014). Use of Self-Complementary Adeno-Associated Virus Serotype 2 as a Tracer for Labeling Axons: Implications for Axon Regeneration. PLoS ONE, 9(2), e87447. https://doi.org/10.1371/journal.pone.0087447

Eom, K., Kim, J., Choi, J. M., Kang, T., Chang, J. W., Byun, K. M., … Kim, S. J. (2014). Enhanced infrared neural stimulation using localized surface plasmon resonance of gold nanorods. Small (Weinheim an Der Bergstrasse, Germany), 10(19), 3853–3857. https://doi.org/10.1002/smll.201400599

Bawin, T., Boukraa, S., Seye, F., Raharimalala, F. N., Zimmer, J. Y., Delvigne, F., & Francis, F. (2014). Using micro-injection technique to assess fungal toxicity in mosquito control. Communications in Agricultural and Applied Biological Sciences, 79(1), 181–185. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/25864335

Tyan, L., Chamberland, S., Magnin, E., Camiré, O., Francavilla, R., David, L. S., … Topolnik, L. (2014). Dendritic Inhibition Provided by Interneuron-Specific Cells Controls the Firing Rate and Timing of the Hippocampal Feedback Inhibitory Circuitry. Journal of Neuroscience, 34(13).

Park, D.-Y., Lee, J., Park, I., Choi, D., Lee, S., Song, S., … Koh, G. Y. (2014). Lymphatic regulator PROX1 determines Schlemm’s canal integrity and identity. The Journal of Clinical Investigation, 124(9), 3960–3974. https://doi.org/10.1172/JCI75392

Fukuyo, Y., Nakamura, T., Bubenshchikova, E., Powell, R., Tsuji, T., Janknecht, R., & Obara, T. (2014). Nephrin and Podocin functions are highly conserved between the zebrafish pronephros and mammalian metanephros. Molecular Medicine Reports, 9(2), 457–465. Retrieved from http://www.spandidos-publications.com/mmr/9/2/457/abstract

Chen, X.-G., Wang, Y.-H., Wen, C.-C., & Chen, Y.-H. (2014). Overdose of D-serine Induces Movement Disorder and Neuromuscular Changes of Zebrafish Larvae. Journal of Toxicologic Pathology, 27(1), 19–24. https://doi.org/10.1293/tox.2013-0032

Rösch, S., Johnen, S., Mataruga, A., Müller, F., Pfarrer, C., Walter, P., … S, N. (2014). Selective Photoreceptor Degeneration by Intravitreal Injection of N-Methyl-N-Nitrosourea. Investigative Opthalmology & Visual Science, 55(3), 1711. https://doi.org/10.1167/iovs.13-13242

Aschauer, D. F., Kreuz, S., & Rumpel, S. (2013). Analysis of transduction efficiency, tropism and axonal transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse brain. PloS One, 8(9), e76310. https://doi.org/10.1371/journal.pone.0076310

Obara, T. (2013). Nephrin and Podocin functions are highly conserved between the zebrafish pronephros and mammalian metanephros. Molecular Medicine Reports. https://doi.org/10.3892/mmr.2013.1844

Ichimura, K., Powell, R., Nakamura, T., Kurihara, H., Sakai, T., & Obara, T. (2013). Podocalyxin regulates pronephric glomerular development in zebrafish. Physiological Reports, 1(3). https://doi.org/10.1002/phy2.74

Socha, M. J., & Segal, S. S. (2013). Isolation of microvascular endothelial tubes from mouse resistance arteries. Journal of Visualized Experiments : JoVE, (81), e50759. https://doi.org/10.3791/50759

Ohana, E., Shcheynikov, N., Moe, O. W., & Muallem, S. (2013). SLC26A6 and NaDC-1 transporters interact to regulate oxalate and citrate homeostasis. Journal of the American Society of Nephrology : JASN, 24(10), 1617–1626. https://doi.org/10.1681/ASN.2013010080

Jørgensen, S. T., Eriksen, H. N., Dausell, J., Jespersen, T., Lambert, I. H., & Hoffmann, E. K. (2013). Double mutation at the putative protein kinase C phosphorylation sites Thr151 plus Thr323 in the mouse leukotrieneD4 receptor eliminates homologous desensitization. Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology, 31(2–3), 366–378. https://doi.org/10.1159/000343374

D’hondt, C., Himpens, B., & Bultynck, G. (2013). Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells. Journal of Visualized Experiments, (77), e50443–e50443. https://doi.org/10.3791/50443

Artegiani, B., Lange, C., & Calegari, F. (2012). Expansion of Embryonic and Adult Neural Stem Cells by <em>In Utero</em> Electroporation or Viral Stereotaxic Injection. Journal of Visualized Experiments, (68), e4093–e4093. https://doi.org/10.3791/4093

Khlghatyan, J., & Saghatelyan, A. (2012). Time-lapse Imaging of Neuroblast Migration in Acute Slices of the Adult Mouse Forebrain. Journal of Visualized Experiments, (67), e4061–e4061. https://doi.org/10.3791/4061

Yu, H., Fischer, G., Jia, G., Reiser, J., Park, F., & Hogan, Q. H. (2011). Lentiviral gene transfer into the dorsal root ganglion of adult rats. Molecular Pain, 7(1), 63. https://doi.org/10.1186/1744-8069-7-63

Chiang, C.-L., Chen, S.-S. A., Lee, S. J., Tsao, K.-C., Chu, P.-L., Wen, C.-H., … Lee, H. (2011). Lysophosphatidic acid induces erythropoiesis through activating lysophosphatidic acid receptor 3. Stem Cells (Dayton, Ohio), 29(11), 1763–1773. https://doi.org/10.1002/stem.733

Guo, Q., Jin, S., Wang, X.-L., Wang, R., Xiao, L., He, R., & Wu, Y. (2011). Hydrogen sulfide in the rostral ventrolateral medulla inhibits sympathetic vasomotor tone through ATP-sensitive K+ channels. The Journal of Pharmacology and Experimental Therapeutics, 338(2), 458–465. https://doi.org/10.1124/jpet.111.180711

Liu, C., Pitts, R. J., Bohbot, J. D., Jones, P. L., Wang, G., & Zwiebel, L. J. (2010). Distinct olfactory signaling mechanisms in the malaria vector mosquito Anopheles gambiae. PLoS Biology, 8(8), e1000467. https://doi.org/10.1371/journal.pbio.1000467

Predel, R., Neupert, S., Garczynski, S. F., Crim, J. W., Brown, M. R., Russell, W. K., … Nachman, R. J. (2010). Neuropeptidomics of the mosquito Aedes aegypti. Journal of Proteome Research, 9(4), 2006–2015. https://doi.org/10.1021/pr901187p

Czirják, G., & Enyedi, P. (2010). TRESK background K(+) channel is inhibited by phosphorylation via two distinct pathways. The Journal of Biological Chemistry, 285(19), 14549–14557. https://doi.org/10.1074/jbc.M110.102020

Chen, J., Joon Lee, H., Jakovcevski, I., Shah, R., Bhagat, N., Loers, G., … Schachner, M. (2010). The Extracellular Matrix Glycoprotein Tenascin-C Is Beneficial for Spinal Cord Regeneration. Molecular Therapy, 18(10), 1769–1777. https://doi.org/10.1038/mt.2010.133

Li, B., Predel, R., Neupert, S., Hauser, F., Tanaka, Y., Cazzamali, G., … Park, Y. (2008). Genomics, transcriptomics, and peptidomics of neuropeptides and protein hormones in the red flour beetle Tribolium castaneum. Genome Research, 18(1), 113–122. https://doi.org/10.1101/gr.6714008

Goldberg, M. W., Huttenlauch, I., Hutchison, C. J., & Stick, R. (2008). Filaments made from A- and B-type lamins differ in structure and organization. Journal of Cell Science, 121(Pt 2), 215–225. https://doi.org/10.1242/jcs.022020

The video below gives an overview of the Nanoliter 2010 microinjection pump.